Purified anti-rat CD90 Antibody anti-CD90

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Clone: 2E11 (See other available formats)
Regulatory Status: RUO
Other Names: Thy-1, Thy1
Isotype: Mouse IgG1, κ
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Product Citations: publications

2E11_PURE_CD90_Antibody_FC_072115 — LOU rat thymocytes were stained with purified CD90 (clone 2E11, filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.

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206101

50 µg

£45

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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, and stromal cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: PC12 cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats of this clone) include: immunoprecipitation and Western blotting.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 206104) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg)
RRID: AB_2565895 (BioLegend Cat. No. 206101)

Antigen Details

Structure: Ig superfamily, GPI-anchored membrane glycoprotein.
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, and glomeruli.
Function: Adhesion, signal transduction, lymphocyte co-stimulation, and proliferation and differentiation of hematopoietic stem cells.
Ligand/Receptor: Interacts with CD45.
Cell Type: B cells, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838 View all products for this Gene ID
UniProt: View information about CD90 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD90 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD90	2E11	FC,IP,WB
Ultra-LEAF™ Purified anti-rat CD90	2E11	FC,IP,WB
FITC anti-rat CD90	2E11	FC
Alexa Fluor® 647 anti-rat CD90	2E11	FC,IHC-F

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/22/2015

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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