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Purified anti-RBFOX1 (A2BP1) Antibody

Pricing & Availability
Clone
A18069B (See other available formats)
Regulatory Status
RUO
Other Names
RBFox1, A2BP1, RNA Binding fox-1 Homolog 1, Hexaribonucleotide Binding Protein 1, FOX1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_A18069B_Purified_RBFOX1_Antibody_1_032719.png
Western blot of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system
  • 1_A18069B_Purified_RBFOX1_Antibody_1_032719.png
    Western blot of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B). Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system
  • A18069B_Purified_RBFOX1_Antibody_1_032719.pn6
    IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18069B_Purified_RBFOX1_Antibody_2_032719.png
    IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A18069B_Purified_RBFOX1_Antibody_3_032719.png
    IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A18069B_Purified_RBFOX1_Antibody_4_032719.png
    IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A18069B_Purified_RBFOX1_Antibody_5_032719.png
    IHC staining of purified anti-RBFOX1 (A2BP1) antibody (clone A18069B) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Compare all formats
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862701 25 µg £73
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862702 100 µg £189
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Description

Rbfox1 is a member of the RNA binding protein family that shares a conservative RNA recognition motif. Rbfox1 has been shown to modulate alternative splicing by binding the consensus RNA sequence motif (U)GCAUG within introns. The interaction between cytoplasmic Rbfox1 and mRNA affects mRNA stability and translation. The binding of Rbfox1 protein to the C-terminus of ataxin-2 may be a contributing factor to the development of spinocerebellar ataxia type 2 .

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801247 (BioLegend Cat. No. 862701)
AB_2801247 (BioLegend Cat. No. 862702)

Antigen Details

Structure
RBFOX1 is a 416 amino acid protein with an apparent molecular mass of ~ 45 kD.
Distribution

Tissue Distribution: brain and heart.
Cellular Distribution: nucleus, golgi apparatus, and cytosol.

Function
RBFOX1 is an RNA binding protein that modulates mRNA alternative splicing.
Interaction
RNA, ataxin-2
Cell Type
Neurons, Oligodendrocytes
Biology Area
Neurodegeneration, Protein Synthesis, Transcription Factors
Antigen References
  1. Wang ET, et al. 2016. J. Neurosci. 36(45):11418
  2. Duan W, et al. 2016. Mol Neurobiol. 53(3):1637
  3. Kuroyanagi H, 2009. Cell Mol Life Sci. 66(24):3895
Gene ID
54715 View all products for this Gene ID 268859 View all products for this Gene ID 302920 View all products for this Gene ID
UniProt
View information about RBFOX1 on UniProt.org
Go To Top Version: 2    Revision Date: 01/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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