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Purified anti-RBX1 Antibody

Pricing & Availability
Clone
W19401A (See other available formats)
Regulatory Status
RUO
Other Names
Ring-Box 1, RNF75, ROC1, Regulator of Cullins 1, RING Finger Protein 75, RING-Box Protein 1, Rbx1, E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein ligase RBX1, Protein ZYP
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19401A_PURE_RBX1_Antibody_113022
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-RBX1 (clone W19401A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W19401A_PURE_RBX1_Antibody_113022
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-RBX1 (clone W19401A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W19401A_PURE_RBX1_Antibody_2_112922
    ICC staining of purified anti-RBX1 (clone W19401A) on HeLa cells (panel B) or purified rat IgG2b, κ isotype control (panel A) (Cat. No. 400602). The cells were fixed and permeabilized with 4% paraformaldehyde, permeabilized with 0.5% Triton X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801), and the image was captured with a 60X objective. Scale bar = 50 µm
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607651 25 µg £92
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607652 100 µg £238
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Description

RBX1 is a RING (Really Interesting New Gene) finger-like domain-containing protein. RBX1 is an E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes that mediate ubiquitination and subsequent proteasomal degradation of target proteins, including those involved in cell cycle progression, signal transduction, and transcription. RBX1 also promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant human RBX1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for IHC-P.

RRID
AB_2927959 (BioLegend Cat. No. 607651)
AB_2927959 (BioLegend Cat. No. 607652)

Antigen Details

Structure
RBX1 is a 108 amino-acid protein with a predicted molecular weight of 12 kD.
Distribution

RBX1 is ubiquitously expressed; nucleus or cytoplasm

Interaction
Part of a SCF complex that also includes CUL1, SKP1 and SKP2; part of the DCX of the DCX DET1-COP1 ubiquitin ligase complex that also potentially includes DET1, DDB1, CUL4A and COP1.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Ubiquitin/Protein Degradation
Antigen References
  1. Hao J, et al. 2015. Oncotarget. 6:42028-39.
  2. Ohta T, et al. 1999. Molecular Cell. 3:535-41. 
  3. Wertz IE, et al. 2004. Science. 303:1371-4.
Gene ID
9978 View all products for this Gene ID
UniProt
View information about RBX1 on UniProt.org
Go To Top Version: 1    Revision Date: 10/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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