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Purified anti-RCAN1 Antibody

Pricing & Availability
Clone
A18067A (See other available formats)
Regulatory Status
RUO
Other Names
Regulator of Calcineurin 1, DSCR1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A18067A_PURE_RCAN1_Antibody_070219.png
Western blot of purified anti-RCAN1 antibody (clone A18067A) and isotype control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blots were incubated with 10 µg/mL of clone A18067A or mouse IgG2b overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18067A_PURE_RCAN1_Antibody_070219.png
    Western blot of purified anti-RCAN1 antibody (clone A18067A) and isotype control mouse IgG2b. Lane 1: Molecular weight marker; Lane 2: 20µg of human brain lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate. The blots were incubated with 10 µg/mL of clone A18067A or mouse IgG2b overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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866201 25 µg £73
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866202 100 µg £189
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Description

RCAN1 is a protein that inhibits the calcineurin/NFAT signaling pathway via it’s interaction with calcineruin A. RCAN1 has three different transcript variants, among which RCAN1.4 has been shown to regulate gene expression, cell migration and tubular morphogenesis in endothelial cells. Dysfunction of RCAN1 has been reported to impact the development of central nervous system, and is clinically linked to Down syndrome. It has also been reported that chronic overexpression of RCAN1 leads to formation of neurofibrillary tangles (NFT), and thus may play a role in neurodegenerative diseases such as AD.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human RCAN1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814606 (BioLegend Cat. No. 866201)
AB_2814606 (BioLegend Cat. No. 866202)

Antigen Details

Structure
RCAN1 is a 252 amino acid protein with predicted molecular mass of ~ 28 kD, and observed molecular masses of ~ 35 kD (RCAN1L) and ~24 kD (RCAN1S) by WB.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and Cytosol

Function
RCAN1 inhibits the calcineurin/NFAT signaling pathway
Interaction
RAF1, PPP3CA, PPP3R1, Calcineurin A
Cell Type
Microglia
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction, Synaptic Biology
Antigen References
  1. Porta S, et al. 2007. Hum Mol Genet. 16(9):1039
  2. Torac E, et al. 2014. J Med Life. 7(4):481
  3. Wu Y, et al. 2014, Mol Neurobiol. 50(3):1085
  4. Harris CD, et al. 2005. Cell Mol Life Sci. 62(21):2477
Gene ID
1827 View all products for this Gene ID 54720 View all products for this Gene ID 266766 View all products for this Gene ID
UniProt
View information about RCAN1 on UniProt.org
Go To Top Version: 1    Revision Date: 07/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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