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Purified anti-RING1 Antibody

Pricing & Availability
Clone
P88C12F4 (See other available formats)
Regulatory Status
RUO
Other Names
RING1A, RNF1, Ring Finger Protein 1, E3 Ubiquitin Ligase RING1, EC:2.3.2.27, Really Interesting New Gene 1 Protein, RNF1
Isotype
Mouse IgG1, κ
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Product Citations
publications
P88C12F4_PURE_RING1_Antibody_1_042820
Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RING1 antibody (clone P88C12F4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • P88C12F4_PURE_RING1_Antibody_1_042820
    Whole cell lysates (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a 1.0 µg/mL (1:500 dilution) of purified anti-RING1 antibody (clone P88C12F4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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936701 25 µg £70
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936702 100 µg £174
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Description

RING1 is an E3 ligase that is essential for development in higher eukaryotes and plants. It is a critical component of the Polycomb-group (PcG) multiprotein PRC1-like complex, which acts to transcriptionally repress genes through post- translational modifications that block the assembly of the transcriptional pre-initiation complex. As a component of PRC1, RING1 plays a pivotal role in monoubiquitination of Histone H2A at Lysine 119, which is linked to transcriptional repression of Hox family members.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human RING1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone may cross-react with RING1B/RING2.

?Due to this clone binding to additional proteins of unknown origin by western blot, it was not tested for ICC.

RRID
AB_2861119 (BioLegend Cat. No. 936701)
AB_2861119 (BioLegend Cat. No. 936702)

Antigen Details

Structure
RING1 is a 406 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

RING1 expression is localized to the nucleus.

Function
Ubiquitin-protein ligase
Interaction
RING2/RNF2
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Ubiquitin/Protein Degradation
Molecular Family
Ligases
Antigen References
  1. Akasaka T, et al. 2002. Genes Cells. 7:835-50.
  2. Levine SS, et al. 2002. Mol Cell Biol. 22:6070-8.
  3. Lovering R, et al. 1993. Proc Natl Acad Sci USA. 90:2112-6.
  4. Satijn DP, et al. 1997. Mol Cell Biol. 17:4105-13.
  5. Wang H, et al. 2004. Nature. 431:873-8.
Gene ID
6015 View all products for this Gene ID
UniProt
View information about RING1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/28/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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