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Purified anti-RNA Polymerase II RPB3 Antibody

Pricing & Availability
Clone
1Y26 (See other available formats)
Regulatory Status
RUO
Other Names
RNA polymerase II sub-unit 3, RPB3, POLR2C
Isotype
Mouse IgG1
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Product Citations
publications
1Y26_Purified_RNAPolymeraseIIRPB3_Antibody_WB_011915
Western blot analysis of yeast (saccharomyces cerevisiae) (lane 1) and Jurkat (lane 2). Anti-RNA Polymerase II RPB3 antibody (clone 1Y26) was used and was followed by HRP anti-mouse IgG antibody.
  • 1Y26_Purified_RNAPolymeraseIIRPB3_Antibody_WB_011915
    Western blot analysis of yeast (saccharomyces cerevisiae) (lane 1) and Jurkat (lane 2). Anti-RNA Polymerase II RPB3 antibody (clone 1Y26) was used and was followed by HRP anti-mouse IgG antibody.
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665004 100 µg £221
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Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The RNA polymermase II subunit RPB3 is a component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft and is known to be involved in transcription termination.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Application

WB - Quality tested
ELISA - Verified
ChIP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
Product Citations
  1. Yurko N, et al. 2017. Mol Cell. 68:913. PubMed
  2. Maudlin IE, et al. 2021. RNA Biol. 18:782. PubMed
  3. Neurohr GE, et al. 2019. Cell. 176:1083. PubMed
  4. Jeronimo C, et al. 2019. Cell Rep. 28:1206. PubMed
  5. Ryu HY, et al. 2019. EMBO J. 38:e102003. PubMed
  6. Jeronimo C, et al. 2021. Molecular Cell. 81:3542. PubMed
  7. Collin P, et al. 2019. Mol Cell. 73:655. PubMed
  8. Abraham K, et al. 2016. Nucleic Acids Res. 44: 8870 - 8884. PubMed
RRID
AB_2565221 (BioLegend Cat. No. 665004)

Antigen Details

Structure
RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
854791 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB3 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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