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Purified anti-RORα Antibody

Pricing & Availability
Clone
6G4C18 (See other available formats)
Regulatory Status
RUO
Other Names
Retinoid related orphan receptor alpha, RORA, Nuclear receptor RZR-alpha, Nuclear receptor subfamily 1 group F member 1 (NR1F1), Nuclear receptor ROR-alpha
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
6G4C18_PURE_RORalpha_Antibody_WB_050218
Total lysates (15 µg protein) from EL-4, A20, NIH3T3 and U87-MG cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-RORα antibody, clone 6G4C18 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • 6G4C18_PURE_RORalpha_Antibody_WB_050218
    Total lysates (15 µg protein) from EL-4, A20, NIH3T3 and U87-MG cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-RORα antibody, clone 6G4C18 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
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683102 100 µg £238
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Description

Retinoid related orphan receptor alpha, also known as RORα, is a NR1 Thyroid Hormone Like Receptor. Three different subtypes have been discovered: RORα, RORβ, and RORγ. RORα plays a key role in the development of the cerebellum. The deletion of RORα in mice causes severe impairment in the differentiation of cerebellar Purkinje neurons and results in the "staggerer" phenotype. RORα has been suggested to participate in the regulation of a number of other physiological processes, which include bone formation. Recently, RORα was reported to regulate lymphocyte development and mediate Th17 differentiation synergistically with RORγ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant protein (isoform 1, 101-308 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

RORa has two isoforms, 53 kD and 59 kD, and this antibody recognizes both isoforms.

This antibody does not recognize human.

RRID
AB_2566748 (BioLegend Cat. No. 683102)

Antigen Details

Structure
523 amino acids with a predicted molecular weight of 59 kD. RORs contain four major functional domains: an amino-terminal domain, DNA domain (DBD), a hinge domain, and a ligand-binding domain (LBD).
Distribution

Nucleus.

Function
RORα can bind as a monomer or homodimer to hormone response elements upstream of several genes to enhance the expression of those genes.
Interaction
RORα interacts with p300, beta-catenin, HIF1A, NCOA2, CEBPB, FOXP3, and NRIP1.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Giguere V, et al. 1994. Genes Dev. 8:538.
2. Atkins GB, et al. 1999. Mol. Endocrinol. 13:1550.
3. Dzhagalov I, et al. 2004. 173:2952.
4. Yang XO, et al. 2008. Immunity 28:29.
5. Lee JM, et al. 2012. Mol. Cell 48:572.
6. Solt LA, et al. 2012. Trends Endocrinol Metab. 23:619.

Gene ID
6095 View all products for this Gene ID
UniProt
View information about RORalpha on UniProt.org
Go To Top Version: 2    Revision Date: 05/03/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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