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Purified anti-RPS27A Antibody

Pricing & Availability
Clone
A18156E (See other available formats)
Regulatory Status
RUO
Other Names
Polyubiquitin-C, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin, ubiquitin B
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A18156E_PURE_RPS27A_Antibody_1_062323
IHC staining of purified anti-RPS27A (clone A18156E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (magenta) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • A18156E_PURE_RPS27A_Antibody_1_062323
    IHC staining of purified anti-RPS27A (clone A18156E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 5 μg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG (magenta) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • A18156E_PURE_RPS27A_Antibody_2_070523
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-RPS27A (clone A18156E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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614451 25 µg £93
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614452 100 µg £281
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Description

Ubiquitin is a small (8.5 kD) regulatory protein that is ubiquitously expressed in tissues of eukaryotic organisms. There are four genes in the human genome that produce ubiquitin: UBB, UBC, UBA52, and RPS27A. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB and UBC genes code for polyubiquitin precursor proteins.

Ubiquitination is a post-translational modification where a ubiquitin subunit is attached to a protein. Addition of ubiquitin can signal for degradation via the proteasome, alter cellular location, promote or prevent protein interactions, or affect activity. Ubiquitination is carried out stepwise by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The cascade results in the binding of ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond.

Proteins can be modified either by a single ubiquitin unit (monoubiquitination) or a chain of ubiquitin molecules (polyubiquitination). Proteins destined for degradation by the proteasome are usually ubiquitinated on lysine residues K48 and K29, while other polyubiquitinations (e.g. on K63, K11, K6) and monoubiquitinations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

A frameshift mutation in ubiquitin B can result in a truncated peptide missing the C-terminal glycine. This abnormal peptide, known as UBB+1, has been shown to accumulate selectively in Alzheimer's disease and other tauopathies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human RPS27A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

WB - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in ICC. For immunohistochemistry, antigen retrieval with sodium citrate is recommended. Clone A18156E is equivalent to BioLegend’s clone P4G7 which detects ubiquitin and ubiquitinylated proteins. The ubiquitin protein is extremely well conserved and thus the antibodies have extensive species cross-reactivity from yeast to human.

RRID
AB_3068138 (BioLegend Cat. No. 614451)
AB_3068138 (BioLegend Cat. No. 614452)

Antigen Details

Structure
RPS27A is a 156 amino acid S27a ribosomal protein fused with a single copy of ubiquitin.
Distribution

Found in almost all tissues of eukaryotic organisms

Function
Regulatory protein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Autophagosome Markers
Antigen References
  1. Hatanaka H, et al. 2009. J. Biol. Chem. 284:15448-57.
  2. Sarkari F, et al. 2013. J. Biol. Chem. 288:16975-16985.
Gene ID
6233 View all products for this Gene ID
UniProt
View information about RPS27A on UniProt.org
Go To Top Version: 1    Revision Date: 06/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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