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Purified anti-RPS6KB1 Antibody

Pricing & Availability
Clone
W17145A (See other available formats)
Regulatory Status
RUO
Other Names
Ribosomal protein S6 kinase beta-1, S6K1, 70 kD ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A (STK14A), p70 ribosomal S6 kinase alpha (p70 S6KA)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17145A_Purified_RPS6KB1_1_WB_Antibody_072418
Total cell lysates (15 µg protein) from HeLa, HEK293, MCF-7 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:2000 (0.25 µg/mL) diluted purified anti-RPS6KB1 antibody (clone W17145A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower panel). Lane M is the MW ladder.
  • W17145A_Purified_RPS6KB1_1_WB_Antibody_072418
    Total cell lysates (15 µg protein) from HeLa, HEK293, MCF-7 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose membrane, and probed with 1:2000 (0.25 µg/mL) diluted purified anti-RPS6KB1 antibody (clone W17145A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower panel). Lane M is the MW ladder.
  • W17145A_Purified_RPS6KB1_2_IP_Antibody_072418
    Total cell lysates (400 µg protein) from HEK293 cells were incubated with 2.5 µg rat IgG2b isotype control (lane 1) or anti-RPS6KB1 antibody (lane 2) at 4°C for 3 hours, followed by incubating the Protein-G beads overnight. Immunoprecipitates and total protein (10% of input, lane 3) were eluted with SDS loading buffer and resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RPS6K1 antibody (clone W17145A). Proteins were visualized using 1:3000 diluted HRP goat anti-rat IgG secondary antibody (Cat. No. 405405) and chemiluminescence detection. Lane M is the MW ladder. IgGHC: IgG heavy chain, IgGLC: IgG light chain.
  • W17145A_Purified_RPS6KB1_3_ICC_Antibody_072418
    MCF-7 cells were fixed with 4% PFA for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) 1: 250 (2 µg/ml) purified rat IgG2b Isotype Ctrl antibody or 1:250 (2 µg/ml, B) and 1:500 (1 µg/ml, C) anti-RPS6KB1 antibody (clone W17145A) overnight at 4 degree followed by Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The images were captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
609901 25 µg £70
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609902 100 µg £174
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Description

Ribosomal protein S6 kinase beta-1 (RPS6KB1), also known as p70S6K, is a serine/threonine kinase participating in the PI3K/mTOR signaling pathway. Upon activation, RPS6KB1 phosphorylates ribosomal protein S6, resulting in selective translation of the 5’-terminal oligopyrimidine tract mRNAs encoding ribosomal proteins and elongation factors. RPS6KB1 also regulates translation initiation and elongation by phosphorylating eukaryotic translation initiation factor 4B (eIF4B) and eukaryotic elongation factor 2 kinase (EEF2K). RPS6KB1 has been found to be crucial in cell growth, cell cycle progression, and cell survival. Inhibition of RPS6KB1 activity results in G1 arrest and delay of entering S phase. Phosphorylation of Bcl-2-associated death promoter (BAD) by RPS6KB1 rescues cells from apoptosis. Impaired regulation of RPS6KB1 has been linked to a variety of diseases, including cancer, diabetes, and obesity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human RPS6KB1 peptide (508-525 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml (1:500 - 1:4000 dilution). For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/ml (1:500 - 1:1000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg for 400 µg total. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

As per the Human Protein Atlas RNA level dataset, HeLa and HEK293 cells are expected to have a low level of RPS6K1 expression.

This clone recognizes two isoforms (56 and 59kD) of RPS6K1 by WB analysis.

RRID
AB_2749938 (BioLegend Cat. No. 609901)
AB_2749938 (BioLegend Cat. No. 609902)

Antigen Details

Structure
525 amino acids with a predicted molecular weight of 59 kD. Contains a serine/threonine kinase domain.
Distribution

Nucleus. Mitochondrion.

Function
RPS6KB1 is a serine/threonine kinase mediating in the PI3K/mTOR signaing pathway. It participates in the regulation of cell growth, cell cycle progression, and cell survival.
Interaction
Interacts with PPP1R9A, RPTOR, IRS1, EIF3B, EIF3C, TRAF4, POLDIP3, and IER5.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Chen B, et al. 2017. PLoS One. 12:e0182891.
  2. Cai C, et al. 2015. Clin Cancer Res. 21:4922.
  3. Bahrami-B F, et al. 2014. J Clin Pathol. 67:1019.
  4. Tao T, et al. 2014. Tumour Biol. 35:8543.
  5. Li PD, et al. 2012. Med Oncol. 29:3070.
Gene ID
6198 View all products for this Gene ID
UniProt
View information about RPS6KB1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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