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Purified anti-SDHB Antibody

Pricing & Availability
Clone
W19244A (See other available formats)
Regulatory Status
RUO
Other Names
Iron-Sulfur Subunit of Complex II, Succinate Dehydrogenase [Ubiquinone] Iron-Sulfur Subunit, SDHIP, SDH2, PGL4, CWS2, Succinate Dehydrogenase Complex Subunit B
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19244A_PURE_SDHB_Antibody_1_112922
IHC staining using purified anti-SDHB (clone W19244A) (panel B) or secondary-only control (panel A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA buffer the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar = 50 µm
  • W19244A_PURE_SDHB_Antibody_1_112922
    IHC staining using purified anti-SDHB (clone W19244A) (panel B) or secondary-only control (panel A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA buffer the tissue was incubated with 10 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar = 50 µm
  • W19244A_PURE_SDHB_Antibody_2_112922
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-SDHB (clone W19244A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W19244A_PURE_SDHB_Antibody_3_112922
    PC-3 cells were fixed with ice cold methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with purified rat IgG2a, κ isotype control (Cat. No. 400502) (Panel A) or 5.0 µg/mL purified anti-SDHB (clone W19244A) (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar = 50 µm
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608751 25 µg £107
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608752 100 µg £238
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Description

SDHB encodes one of the four proteins that form the SDH (succinate dehydrogenase) enzyme complex in the mitochondrial-respiratory chain, and plays an important role in the mitochondria. The SDH complex functions to couple the conversion of succinate to fumarate in the mitochondrial matrix as part of the Krebs cycle with the reduction of ubiquitin to ubiquinol as part of the ETC (electron transport chain). Within the complex itself SDHB provides an attachment site for released electrons to be transferred to the oxidative phosphorylation pathway. Sporadic and familial mutations in this gene result in paragangliomas, pheochromocytoma, and gastrointestinal stromal tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SDHB
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W19244A is suitable for ICC using methanol only as the fixation/permeabilization method. Alternate methods such as fixation with PFA followed by permeabilization with either methanol or Triton-X-100 resulted in staining in the wrong localization.

RRID
AB_2927962 (BioLegend Cat. No. 608751)
AB_2927962 (BioLegend Cat. No. 608752)

Antigen Details

Structure
SDHB is a 280 amino-acid protein with a predicted molecular weight of 32 kD.
Distribution

SDHB is present in the mitochondria.

Function
SDHB provides an attachment site for electrons as they are transferred to the oxidative phosphorylation pathway.
Interaction
SDHB forms the SDH (succinate dehydrogenase) enzyme complex along with SDHA, SDHC, and SDHD.
Biology Area
Cell Biology, Mitochondrial Function, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators, Mitochondrial Markers, Tumor Suppressors
Antigen References
  1. Seedat F, et al. 2021. SDHB Gene Mutation Associated Paraganglioma Syndrome -A Review. 4:118.
  2. Gill AJ. 2012. Pathology. 44:285-92.
Gene ID
6390 View all products for this Gene ID
UniProt
View information about SDHB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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