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Purified anti-Serpin A12 Antibody

Pricing & Availability
Clone
A16088B (See other available formats)
Regulatory Status
RUO
Other Names
Vaspin, Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose-specific serpin, OL-64
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A16088B_PURE_Serpin-A12_IHC-P_091523
IHC staining purified anti-Serpin A12 (clone A16088B) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated with (panel A) and without (panel B) 10 µg/mL of purified anti-Serpin A12 (clone A16088B) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were with a 40X objective. Scale bar: 50 µm
  • A. A16088B_PURE_Serpin-A12_IHC-P_091523
    IHC staining purified anti-Serpin A12 (clone A16088B) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated with (panel A) and without (panel B) 10 µg/mL of purified anti-Serpin A12 (clone A16088B) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were with a 40X objective. Scale bar: 50 µm
  • B. A16088B_PURE_Serpin-A12_WB-1_091523
    Whole cell extracts (15 µg total protein) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-Serpin A12 (clone A16088B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • C. A16088B_PURE_Serpin-A12_WB-2_091523
    Whole cell extracts (15 µg total protein) spiked with 20 ng Recombinant Human Serpin A12 (Cat. No. 756806) or Serpin E1 (Cat. No. 753804) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-Serpin A12 (clone A16088B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Direct-Blot™ HRP anti-His Tag (Cat. No. 362614) was used as spiked recombinant protein control at a 1:500 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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624651 25 µg £103
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624652 100 µg £240
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Description

Human Serpin A12, also known as Vaspin, is a secreted adipokine that is found in visceral adipose tissue. It belongs to the serpin superfamily, class A. It's known protease target is KLK7, a member of the kallikrein family. In humans, Serpin A12 expression may increase at the onset of diabetes and decrease when the disease aggravates. The administration of recombinant Serpin A12 in a diet-induced mouse obesity model suggests that it may represent an insulin-sensitizing adipokine. Low serum concentration of Serpin A12 may be a risk factor for the progression of type-2 diabetes mellitus. Although its mechanism of action is not fully understood, KLK7 inhibition seems to be the most likely underlying physiological mechanism for the compensatory effects on obesity-induced insulin resistance. In addition to its relation to diabetes, Serpin A12 exerts protective effects on heart disease. Its low concentrations correlate with coronary artery diseases’ severity and unstable angina pectoris. Serpin A12 may inhibit endothelial cell apoptosis, attenuate high glucose stimulated vascular smooth muscle cell proliferation, and chemokinesis. It also acts as a ligand for the cell surface GRP78/voltage dependent anion channel complex in endothelial cells and promotes proliferation, inhibits apoptosis, and protects against diabetes mellitus associated vascular injuries.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human Serpin A12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunohistochemistry, Citrate buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended. 

RRID
AB_3083413 (BioLegend Cat. No. 624651)
AB_3083413 (BioLegend Cat. No. 624652)

Antigen Details

Structure
Monomer
Distribution

Expressed in visceral adipose tissues and liver. Possibly present in adipocytes. Present in the plasma and serum

Function
Adipokines that modulate insulin action by specifically inhibiting target protease KLK7 in white adipose tissues
Interaction
KLK7, GRP78/VDAC complex
Biology Area
Cardiovascular Biology, Signal Transduction
Antigen References
  1. Li HL, et al. 2011. Clin Chem Lab Med. 49:1547-54.
  2. Jung CH, et al. 2011. Biochem. Biophys. Res. Commun. 413:264-9.
  3. Li H, et al. 2013. Atherosclerosis. 228:61-8.
  4. Nakatsuka A, et al. 2012. Diabetes. 61:2823-32.
  5. Heiker JT, et al. 2013. Cell Mol. Life Sci. 70:2569-83.
  6. Hida K, et al. 2005. Proc. Natl. Acad. Sci. USA. 30:10610.
  7. Jian W, et al. 2014. PLoS One. 9:e94763.
Gene ID
145264 View all products for this Gene ID
UniProt
View information about Serpin A12 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/15/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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