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Purified anti-Shiga toxin Stx2A Antibody

Pricing & Availability
Clone
1C6A6G9 (See other available formats)
Regulatory Status
RUO
Other Names
SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stxA2, stx2A
Isotype
Mouse IgG1, κ
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Product Citations
publications
1C6A6G9_Pure_ShigaToxin_Antibody_112117
Total protein (15 µg) from bacteria transformed with plasmid containing STX2A without IPTG induction (lane 1) and with IPTG induction ( lane 2) was resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Shiga toxin Stx2A antibody (clone 1C6A6G9) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Ponceau S staining (lower) was used as loading control. M: protein marker.
  • 1C6A6G9_Pure_ShigaToxin_Antibody_112117
    Total protein (15 µg) from bacteria transformed with plasmid containing STX2A without IPTG induction (lane 1) and with IPTG induction ( lane 2) was resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Shiga toxin Stx2A antibody (clone 1C6A6G9) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Ponceau S staining (lower) was used as loading control. M: protein marker.
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665903 25 µg £94
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665904 100 µg £221
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Description

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subnit is cleaved by furin in two fragments: A1, the catalytically active fragment, and A2, the fragment that is essential for holotoxin assembly with the B subunits.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:2500 diluted (0.2 - 1.0 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 1C6A6G9 recognizes epitope 198-212 (REFRQALSETAPVYT).

RRID
AB_2721750 (BioLegend Cat. No. 665903)
AB_2721750 (BioLegend Cat. No. 665904)

Antigen Details

Structure
A is a 35 kD subunit of Shiga toxin.
Biology Area
Bacterial proteins and Toxins, Cell Biology
Gene ID
1262249 View all products for this Gene ID
UniProt
View information about Stx2A on UniProt.org
Go To Top Version: 2    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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