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Purified anti-SHIP-1 Antibody

Pricing & Availability
Clone
P1C1-A5 (See other available formats)
Regulatory Status
RUO
Other Names
Inositol Polyphosphate-5-Phosphatase, SIP-145, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_P1C1dashA5_PURE_SHIP1_Antibody_1_102218
Total cell lysates (15 µg total protein) from PC-3 (negative control), HeLa (negative control), THP-1 and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SHIP-1 Antibody, clone P1C1-A5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1_P1C1dashA5_PURE_SHIP1_Antibody_1_102218
    Total cell lysates (15 µg total protein) from PC-3 (negative control), HeLa (negative control), THP-1 and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-SHIP-1 Antibody, clone P1C1-A5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 2_P1C1dashA5_PURE_SHIP1_Antibody_IF_082917
    THP-1 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. The cells were then intracellularly stained with either (A) 2 µg/ml anti-mouse IgG1, κ or (B) 2 µg/ml SHIP-1 antibody (clone P1C1-A5) , (C) 0.5 µg/ml SHIP-1 antibody (clone P1C1-A5), or (D) 0.2 µg/ml SHIP-1 antibody (clone P1C1-A5), and incubated overnight at 4°C followed by 2 µg/ml (1:250) Alexa Fluor® 488 (Green) goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (Blue). The image was captured with a 60X objective. Exposure times: (A) 1/5, (B) 1/7.5, (C) 1/7.5, (D) 1/7.5.
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656601 100 µg £141
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Description

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes key signaling lipid PI(3,4,5)P(3) to PI(3,4)P(2). SHIP-1 is located in the cytoplasm and is recruited to the cell surface upon receptor ligation. It plays critical roles in hematopoietic cell proliferation, activation, differentiation, and survival by regulating PI3K and Akt pathways. Aberrant regulation of PI3K signaling can induce autoimmune diseases and cancer formation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human SHIP fusion protein in CFA/IFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 4.0 ug/ml dilution. For immunocytochemistry, the suggested use is 0.5 - 1ug/ml (1:500-1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Specific to human SHIP-1 and cross reactive with mouse SHIP-1.

Product Citations
  1. Hsin JP, et al. 2018. Nat Immunol. 19:1137. PubMed
RRID
AB_2562400 (BioLegend Cat. No. 656601)

Antigen Details

Structure
1189 or 1188 amino acids, predicted molecular weight of 150 kD with the characteristic N-terminal SH2 domain, inositol phosphatase domain, and C-terminal protein interaction domains
Distribution

Cytoplasm and membrane; translocates to the plasma membrane when activated

Function
Negative regulator of hematopoietic cell proliferation and survival
Interaction
Phosphatidylinositol-3,4,5-triphosphate
Ligand/Receptor
SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150, PTPN11 FCGR2A FCGR2B, FCGR3, SRC and TEC et al.
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Condé C, et al. 2011. Biochem. Pharmacol. 82:1320.
2. Parry RV, et al. 2010. Biochim Biophys Acta. 1804:592.
3. Ghansah T, et al. 2012. Oncoimmunology. 1:984.
4. Banh C, et al. 2012. Blood 120:4583.
5. Blunt MD, et al. 2012. Front. Immunol. 3:226.

Gene ID
3635 View all products for this Gene ID
UniProt
View information about SHIP-1 on UniProt.org
Go To Top Version: 2    Revision Date: 05/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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