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Purified anti-SHP-2 Antibody

Pricing & Availability
Clone
W18298A (See other available formats)
Regulatory Status
RUO
Other Names
CFC, NS1, JMML, SHP2, BPTP3, PTP2C, METCDS, PTP-1D, SH-PTP2, SH-PTP3, Syp, Protein Tyrosine Phosphatase Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatase 2
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18298A_PURE_SHP-2_Antibody_112322
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-SHP-2 (clone W18298A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W18298A_PURE_SHP-2_Antibody_112322
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-SHP-2 (clone W18298A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • W18298A_PURE_SHP-2_Antibody_2__120722
    IHC staining of purified anti-SHP-2 (clone W18298A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using 1X Tris-Buffered Saline with Tween-20 (final concentration 0.05M) (Cat. No. 925501), the tissue was incubated with blocking solution (negative control) (panels A and C) or 10 μg/mL of antibody (panels B and D) overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801) (panels A and B), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 μm
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609251 25 µg £96
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609252 100 µg £252
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Description

Src homology-2-containing protein tyrosine phosphatase 2 (SHP-2) protein, encoded by the PTPN11 gene, is a well-known oncogenic protein tyrosine phosphatase. SHP-2 regulates cellular functions by dephosphorylating targets downstream of PD1, EGFRvIII, HGF receptor, and cytokine receptors. Cellular processes controlled by SHP-2 include mitotic cycle, cell growth, differentiation, and oncogenesis. SHP-2 has been recognized as a potential therapeutic target for cancers including breast cancer, lung cancer, leukemia, gastric cancer, and oral cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SHP-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25  - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product cross reacts with mouse cell lines in Western-blot application but has poor selectivity. Clone A20016A is recommended for mouse reactivity.

This product is not recommended for immunocytochemistry.

RRID
AB_2927989 (BioLegend Cat. No. 609251)
AB_2927989 (BioLegend Cat. No. 609252)

Antigen Details

Structure
SHP-2 is a 593 amino acid protein with a molecular mass of 68 kD
Distribution

Cytoplasm and Nucleus

Function
SHP-2 regulates cell proliferation, apoptosis, invasion, and metastasis
Interaction
SHP-2 interacts with PD1, EGFR, HGF receptor, RET, FGFR2, FGFR3, PI3K/AKT, and TLR3 signaling pathways.
Cell Type
Lymphocytes, T cells, Th1
Biology Area
Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Hao C, et al. 2020. Oncogene. 39:7166-7180.
  2. Marie D, et al. 2008. Cell Signal. 20:453-9.
  3. Cheng Kui QU. 2000. Cell Research. 10:279-288.
Gene ID
5781 View all products for this Gene ID
UniProt
View information about SHP-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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