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- Clone
- M1307D09 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD170, Sialic acid-binding Ig-like lectin E, Siglec 9, Siglecl1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Western blot analysis of wild type mouse spleen (lane 1) and Siglec-E knock out mouse spleen (lane 2) using anti-Siglec E antibody (clone M1307D09) under non-reducing conditions. Anti-β actin antibody (clone poly6221) was used as a loading control. Siglec-E knock out mouse spleen tissue was kindly provided by Dr. Ajit Varki, UCSD.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 682802 | 100 µg | £182 | ||||
Siglecs (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. The cytoplasmic tails of all siglecs have tyrosine based motifs with signaling functions. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that siglecs may participate in cell-cell interactions or act as receptors for the entry of viral or bacterial pathogens. Additionally, the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domain indicates that these molecules may play a role in the suppression of immunoreceptor signaling. Siglec-E is a mouse CD33-related siglec that selectively regulates early recruitment of neutrophils to the lungs in an acute lung inflammation induced by lipopolysaccharides. Siglec E-deficient mice exhibited exaggerated neutrophil recruitment that is reversible by using a blockade of the β2 integrin, CD11b. In addition, sialidase treatment of fibrinogen reverses the suppressive effects of Siglec-E on CD11b signaling. This suggests that sialic acid recognition by Siglec-E is required for its inhibitory function. These findings indicate that Siglec-E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse Siglec-E produced in the 293E cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody works for Western blotting under non-reducing conditions.
-
Application References
(PubMed link indicates BioLegend citation) -
- Siddiqui S, et. al. 2017. J. Biol. Chem. 292: 1029.
- Product Citations
-
- RRID
-
AB_2566696 (BioLegend Cat. No. 682802)
Antigen Details
- Structure
- 569 amino acids with a predicted molecular weight of approximately 65 kD.
- Distribution
-
Murine Siglec-E is expressed by neutrophils, natural killer (NK) cells, monocytes, macrophages, dendritic cells, and B1 cells.
- Function
- Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
- Ligand/Receptor
- α2,8-linked disialic acid.
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Siglec Molecules
- Antigen References
-
1. McMillan SJ, et al. 2013. Blood 121:2084.
2. Bax M, et al. 2007. J. Immunol. 12:8216-24.
3. Angata T, Varki A. 2000. J. Biol. Chem. 275:22127.
4. Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
5. Yu Z, et al. 2001. Biochem. J. 353:483. - Gene ID
- 83382 View all products for this Gene ID
- UniProt
- View information about Siglec-E on UniProt.org
Related FAQs
Other Formats
View All Siglec-E Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Siglec-E | M1307D09 | WB,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Siglec-E
Western blot analysis of wild type mouse spleen (lane 1) and...
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