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Purified anti-SIRT1 Antibody

Pricing & Availability
Clone
W16003A (See other available formats)
Regulatory Status
RUO
Other Names
NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1 (SIR2L1), Regulatory protein SIR2 homolog 1, hSIRT1, hSIR2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16003A_PURE_SIRT1_Antibody_WB_110117
Total lysates (10µg protein) from HeLa and HeLa SIRT1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-SIRT1 antibody , clone W16003A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-rat-IgG secondary antibody (cat#405405). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 1_W16003A_PURE_SIRT1_Antibody_WB_110117
    Total lysates (10µg protein) from HeLa and HeLa SIRT1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-SIRT1 antibody , clone W16003A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-rat-IgG secondary antibody (cat#405405). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 2_W16003A_PURE_SIRT1_Antibody_WB_090616
    Total cell lysate from human HeLa cells (lane 1), 293T cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SIRT1 (clone W16003A) antibody. Proteins were visualized using an HRP goat anti-rat IgG antibody and chemiluminescence detection. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
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690502 100 µg £174
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Description

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuates p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human SIRT1 recombinant protein (548-747 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629822 (BioLegend Cat. No. 690502)

Antigen Details

Structure
747 amino acids with a predicted molecular weight of 81.6 kD and observed of approximately 120 kD. Contains a sirtuin-type deacetylase domain.
Distribution

Nucleus, cytoplasm, and PML body.

Function
SIRT1 is a NAD-dependent deacetylase involving in the regulation of cellular metabolism and stress responses.
Interaction
Interacts with p53, E1A binding protein p300, HEY2, NF-kB3, Transcription factor HES-1, Forkhead box protein O3A, and PML.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Li L, Bhatia R. 2015. Curr. Opin. Hematol. 22:324.
2. Qiu G, et al. 2015. FEBS Lett. 589:2034.
3. Chen X, et al. 2015. Immunology 145:455.
4. Simmons GE Jr, et al. 2015. Int. J. Mol. Sci. 16:950.
5. Kauppinen A, et al. 2013. Cell Signal. 25:1939.
6. Paraíso AF, et al. 2013. Mol. Neurobiol. 48:681.

Gene ID
23411 View all products for this Gene ID
UniProt
View information about SIRT1 on UniProt.org
Go To Top Version: 2    Revision Date: 11/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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