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- Clone
- O91G9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sirtuin 5, SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2) and 20nM SIRT5 siRNA (Lane 3) treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-SIRT5 antibody (upper). Proteins were visualized using 1:3000 diluted HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted) was used as a loading control (lower). -
Total cell lysate (15µg protein) from THP-1, HeLa and Raw264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/mL), 1:1000 (0.5µg/mL) and 1:2500 (0.2µg/mL) diluted purified anti-SIRT5 antibody (clone O91G9) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody for clone O91G9 or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder, M* indicates longer exposure.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 698801 | 25 µg | £70 | ||||
| 698802 | 100 µg | £174 | ||||
The seven mammalian Sirtuin isoforms are assigned to different Sirtuin classes on the basis of sequence homology. The mitochondrial SIRT5 belongs to class III. In the mitochondrial matrix, Sirt5 deacetylates and activates carbamoyl phosphate synthetase 1 (CPS1), which is increased when amino acid metabolism is upregulated during fasting. More importantly, SIRT5 regulates lysine succinylation and malonylation as evolutionarily conserved post-translation modifications. SIRT5 was proved to regulate many metabolic pathways, with fatty acid beta-oxidation and ketone body production as two pathways strongly enriched in lysine succinylation. Recently, Zhang et al demonstrated that SIRT5 was targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin in order to promote respiratory chain function.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length protein expressed in 293T cell transfected with human SIRT5 expression vector.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
SiRNA knockdown (KD) was used to confirm specificity of this antibody.
- RRID
-
AB_2687321 (BioLegend Cat. No. 698801)
AB_2687321 (BioLegend Cat. No. 698802)
Antigen Details
- Structure
- 310 amino acids, and predicted Mw is 34kDa
- Distribution
-
Mitochondrion
- Function
- NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically remove malonyl, succinyl and glutaryl groups on target proteins.
- Interaction
- Interacts with CPS1, cardiolipin. Forms homodimers upon suramin binding.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
-
1. Wegner M. 1999. Nucleic Acids Res. 27: 1409.
2. Gertz M, et al. 2016. Cell. Mol. Life Sci. 73:2871.
3. Osborne B, et al. 2016. Free Radic. Biol. Med. 100:164.
4. Nakagawa T, et al. 2009. Cell 137:560.
5. Peng, et al. 2011. Mol. Cell. Proteomics 10:M111.012658.
6. Guan KL and Xiong Y. 2010. Trends Biochem. Sci. 36: 108.
7. Rardin MJ, et al. 2013. Cell. Metab. 18:920.
8. Zhang Y, et al. 2017. J. Biol. Chem. M117.785022. - Gene ID
- 23408 View all products for this Gene ID
- UniProt
- View information about SIRT5 on UniProt.org
Related FAQs
Other Formats
View All SIRT5 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-SIRT5 | O91G9 | WB,KO/KD-WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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