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Purified anti-Skp1 Antibody

Pricing & Availability
Clone
W18108A (See other available formats)
Regulatory Status
RUO
Other Names
S-Phase Kinase Associated Protein 1 , Transcription Elongation Factor B Polypeptide 1-Like, Cyclin-A/CDK2-Associated Protein P19, Organ Of Corti Protein II, P19skp1, OCP-II, TCEB1L, SKP1A, EMC19, SIII,RNA Polymerase II Elongation Factor-Like Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18108A_PURE_Skp1_Antibody_1_120419
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Skp1 antibody (clone W18108A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18108A_PURE_Skp1_Antibody_1_120419
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Skp1 antibody (clone W18108A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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669801 25 µg £70
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669802 100 µg £174
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Description

S-phase kinase-associated protein 1 (Skp1) is one of the components of the SCF complex, which belongs to the E3 ubiquitin-protein ligase family. The SCF complex recruits substrates and promotes ubiquitin-mediated degradation of the substrates. Skp1 forms an invariable core component of the SCF complex with Cul1 and Rbx1 proteins. Skp1 binds to F-box proteins, which recruit the substrates for proteolysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant human Skp1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes human Skp1 and cross-reacts with mouse Skp1.

When tested for WB, this clone detected a ~40 kDa protein of unknown origin; this band was not observed at lower antibody titrations.

Because of the binding of this antibody to the unknown protein, this clone was not tested for ICC.

This clone failed IP testing.

RRID
AB_2820110 (BioLegend Cat. No. 669801)
AB_2820110 (BioLegend Cat. No. 669802)

Antigen Details

Structure
Skp1 is a 163 amino acid protein with a predicted molecular weight of 19 kD.
Distribution

Centrosome/Cytosol/Nucleus/Ubiquitously expressed

Function
Adaptor protein
Biology Area
Cell Biology, Ubiquitin/Protein Degradation
Molecular Family
Adaptor Proteins
Antigen References
  1. Bai C, et al. 1996. Cell. 86:263
  2. Zheng N, et al. 2002. Nature. 416:703
  3. Schulman B, et al. 2000. Nature. 408:381
Gene ID
6500 View all products for this Gene ID
UniProt
View information about Skp1 on UniProt.org
Go To Top Version: 1    Revision Date: 12/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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