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Purified anti-SMC3 Antibody

Pricing & Availability
Clone
DJ5.2.3 (See other available formats)
Regulatory Status
RUO
Other Names
Structural maintenance of chromosomes protein 3 (SMC3), Basement membrane-associated chondroitin proteoglycan (BAMACAN), Chondroitin sulfate proteoglycan 6 (CSPG6), Chromosome-associated polypeptide (HCAP)
Isotype
Mouse IgG1, κ
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Product Citations
publications
DJ523_PURE_SMC3_Antibody_041122
Whole cell extracts (15 µg total protein) from the indicated cell lines were boiled at 100°C for 5 minutes and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL of purified anti-SMC3 (clone DJ5.2.3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • DJ523_PURE_SMC3_Antibody_041122
    Whole cell extracts (15 µg total protein) from the indicated cell lines were boiled at 100°C for 5 minutes and were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL of purified anti-SMC3 (clone DJ5.2.3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
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605351 25 µg £81
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605352 100 µg £230
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Description

Structural maintenance of chromosomes protein 3 (SMC3) is a member of the SMC protein family and a component of the cohesin complex. Cohesin plays an important role in mitosis and meiosis by mediating sister chromatid cohesion, homologous recombination, and spindle pole formation. SMC3 activity is mediated by phosphorylation at a pair of conserved lysine residues, stabilizing the cohesion complex during mitosis. Mutations in SMC3 can result in Cornelia de Lange syndrome, characterized by developmental disabilities.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Reported Reactivity
X. laevis
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic fragment of X. laevis SMC3, raised from mice immunized with the peptide sequence: EEVSLRRVIGAKKDQYFLDKK, corresponding to amino acids 94 -114 of Xenopus laevis SMC3.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Product was tested for use in ICC and IHC-P applications but did not meet passing criteria.

RRID
AB_2924611 (BioLegend Cat. No. 605351)
AB_2924611 (BioLegend Cat. No. 605352)

Antigen Details

Structure
SMC3 is a 1217 amino acid protein with a predicted molecular weight of 141.5 kD.
Distribution

SMC3 is present in the nucleus of cells. During mitosis, it segregates exclusively at the centrosomes of chromosomes. 

Interaction
SMC3 is a component of the cohesion complex consisting SMC1, SCC1, and SCC3
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Beckouet F, et al. 2010. Mol Cell. 39:689-99. 
  2. Boyle MI, et al. 2015. Clin Genet. 88:1-12. 
  3. Gligoris TG, et al. 2014. Science. 346:963-7. 
Gene ID
9126 View all products for this Gene ID
UniProt
View information about SMC3 on UniProt.org
Go To Top Version: 1    Revision Date: 04/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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