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Purified anti-SNAIL Antibody

Pricing & Availability
Clone
W22252A (See other available formats)
Regulatory Status
RUO
Other Names
SNAI1, Snail Family Transcriptional Repressor, SNAH, SLUGH2, SNAIL, SNA, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22252A_PURE_SNAIL_WB_102824
Whole cell lysates (15 µg total protein) from indicated cell lines were resolved by gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SNAIL (clone W22252A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22252A_PURE_SNAIL_WB_102824
    Whole cell lysates (15 µg total protein) from indicated cell lines were resolved by gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-SNAIL (clone W22252A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22252A_PURE_SNAIL_WBICC_102824
    MCF7 cells (low expressing cell line, panel A) and BeWo cells (high expressing cell line, panel B) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized with 0.5% Triton X-100, and blocked with 5% FBS. Cells were then stained with Purified anti-SNAIL (clone W2222A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm.
  • C. W22252A_PURE_SNAIL_ICFC_102824
    BeWo cells (high expressing cell line, filled histogram) and MCF-7 cells (low expressing cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-SNAIL (clone W22252A) or purified rat IgG2b, κ Isotype Control (open histogram, dashed line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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673251 25 µg £124
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673252 100 µg £325
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Description

SNAIL is a zinc finger transcriptional repressor that is involved in cell movement, adhesion, and survival. SNAIL plays a pivotal role in the epithelial to mesenchymal transition (EMT) during embryonic development. SNAIL represses the expression of E-cadherin in epithelial cells leading to the disassembly of cell-cell junctions, loss of cell polarity, and increased migration. In pathological contexts, SNAIL can promote tumor progression and metastasis by allowing cancer cells to invade surrounding tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SNAIL protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody in immunocytochemistry (ICC), we recommend fixation/permeabilization with PFA 2% and Triton X-100 0.5%. We do not recommend PFA/methanol or methanol alone.

Antigen Details

Structure
SNAIL is a 264 amino acid protein with a molecular weight of approximately 29 kDa.
Distribution

SNAIL is primarily located in the nucleus of the cell but can be transported to the cytoplasm upon phosphorylation.

Function
EMT, embryonic development, cell migration
Interaction
FBXL14, GSK3B, BTRC, WTIP, LIMD1 LOXL2 and LOXL3, CSNK2A1, EGR1, LATS2, KPNB1, TNPO1, KPNA1, KPNA4, IPO7, PARP1, KDM1A, p53, NOTCH1, MDM2, CSNK1E, and PAK1
Antigen References
  1. Dong B, et al. 2021. Int J Mol Sci. 22:11062.
  2. Olmeda D, et al. 2007. Cancer Res. 67:11721.
  3. Baulida J, et al. 2019. J Clin Med. 8: 757.
  4. Suzuki T, et al. 2023. J Cancer Metastasis Treat. 9:25.
  5. Sun JX, et al. 2018. Angiogenesis. 21:635.
Gene ID
6615 View all products for this Gene ID
UniProt
View information about SNAIL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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