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Purified anti-SOX17 Recombinant Antibody

Pricing & Availability
Clone
O92B6.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor SOX17, Transcription factor SOX 17, Transcription factor SOX-17, VUR3, SRY-Box17, SRY-Related HMG-Box Transcription Factor 17
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_O92B6ptRec_PURE_SOX17_Recom_Antibody_WB_122123
Whole cell extracts (15 μg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of Purified anti-SOX17 Recombinant (clone O92B6.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A_O92B6ptRec_PURE_SOX17_Recom_Antibody_WB_122123
    Whole cell extracts (15 μg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL of Purified anti-SOX17 Recombinant (clone O92B6.Rec) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B_O92B6ptRec_PURE_SOX17_Recom_Antibody_ICC_122123
    HepG2 cells (negative cell line, panel A) and OVCAR-3 cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 μg/mL of Purified anti-SOX17 Recombinant (clone O92B6.Rec), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 40X objective. Scale bar: 50 μm
  • C_O92B6ptRec_PURE_SOX17_Recom_Antibody_IHC_122123
    IHC staining of Purified anti-SOX17 Recombinant (clone O92B6.Rec) on formalin-fixed paraffin-embedded human endometrium tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated with 10 μg/mL of Purified anti-SOX17 Recombinant (clone O92B6.Rec) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panels A and B) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (Panel A). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 40X objective. Scale bar: 50 μm
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628151 25 µg £130
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628152 100 µg £325
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Description

SOX17 is an important member of the SOX family of transcription factors, which are defined by possession of a homologous sequence called the HMG (high mobility group) box. SOX genes are primarily involved in developmental processes including germ layer formation, cell-type specification, and organogenesis. SOX17 activates endodermal target genes, and is required for the formation of endoderm and blood vessel endothelium. SOX17 also plays critical roles in the maintenance of fetal and neonatal hematopoietic stem cells. Additionally, SOX17 is a regulator of human primordial germ cell fate.

SOX17 also acts as a tumor suppressor through suppression of Wnt signaling, and a reduction of SOX17 expression is found in gastrointestinal and colon carcinomas. Additionally, mutations in SOX17 can induce stem-like characteristics in tumor cells by causing SOX17 to dimerize with OCT4 and induce pluripotency transcriptional programs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant protein expressed in E. Coli corresponding to residues 177-414 of human SOX17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50 mM sodium citrate
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone O92B6.Rec) does not cross-react with mouse SOX17 in western blot (WB).

For immunohistochemistry (IHC), we recommend antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) or Sodium Citrate (Cat. No. 928502).

For immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 100% ice-cold methanol or 0.5% Triton-X. Fixation and permeabilization with 100% ice-cold methanol alone is not recommended.

RRID
AB_3097533 (BioLegend Cat. No. 628151)
AB_3097533 (BioLegend Cat. No. 628152)

Antigen Details

Structure
SOX17 is a 414 amino acid protein with a predicted molecular weight of 44.1 kD.
Distribution

Nucleus

Function
Transcription factor
Interaction
Interacts with CTNNB1, LEF1, TCF4
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References
  1. Wegner M. 1999. Nucleic Acids Res. 27:1409-20.
  2. Du YC, et al. 2009. Gastroenterology. 137:1346-57.
  3. Zorn AM, et al. 1999. Mol Cell. 4:487-98.
  4. Sinner D, et al. 2004. Development. 131:3069-80.
  5. Hudson C, et al. 1997. Cell. 91:397-405.
  6. Matsui T, et al. 2006. J Cell Sci. 119:3513-26.
  7. Kim I, et al. 2007. Cell. 130:470-83.
  8. Zhang W, et al. 2008. Cancer Res. 68:2764-72.
  9. Tan DS, et al. 2020. Semin Cancer Biol. 67:65-73.
  10. Irie N, et al. 2015. Cell. 160:253-68.
Gene ID
64321 View all products for this Gene ID
UniProt
View information about SOX17 on UniProt.org
Go To Top Version: 1    Revision Date: 12/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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