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Purified anti-Spastin Antibody

Pricing & Availability
Clone
Sp3G11/1 (See other available formats)
Regulatory Status
RUO
Other Names
Spastic paraplegia 4 protein, SPG4, ADPSP, FSP2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
Sp3G11-1_PURE_Spastin_Antibody_1_070919
Western blot of purified anti-Spastin antibody (clone Sp3G11/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Sp3G11-1_PURE_Spastin_Antibody_1_070919
    Western blot of purified anti-Spastin antibody (clone Sp3G11/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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868201 25 µg £73
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868202 100 µg £189
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Description

Spastin is a microtubule-severing protein that cleaves longer microtubules (MTs) to shorter ones. The severing of MT regulates its numbers and mobility, and the distribution of the plus-end. This has also been related to membrane trafficking of microtubules. Mutations in the spastin gene SPG4 is the main cause for hereditary spastic paraplegia, a neurodegenerative disorder mainly occurring in corticospinal tracks. Due to the presence of 2 start codons in SPG4 gene, 2 isoforms of spastin exist: a 68 kD isoform termed M1 and a 60 kD isoform termed M87. The M1 isoform is only detected in the adult spinal cord while the M87 isoform is ubiquitously expressed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human spastin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814618 (BioLegend Cat. No. 868201)
AB_2814618 (BioLegend Cat. No. 868202)

Antigen Details

Structure
Human spastin is a 616 amino acid protein with a predicted molecular mass of 67 kD.
Distribution

Tissue distribution: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle. The short isoform may predominate in the brain and spinal cord.
Cellular distribution: Cytoskeleton, endoplasmic reticulum, nucleus, and membrane.

Function
Spastin is involved in the regulation of microtubule severing.
Interaction
Spastin mostly forms homohexamer.
Cell Type
Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Hazan J. et al. 1999. Nat Genet. 23(3):296-303.
  2. Solowska JM. et al. 2014. J Neurosci. 34(5):1856-67. 
Gene ID
6683 View all products for this Gene ID
UniProt
View information about Spastin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/09/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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