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Purified anti-STAT1 Antibody

Pricing & Availability
Clone
A15158C (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 1, Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
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Product Citations
publications
A15158C_Pure_Stat1_Antibody_1_082918
Total cell lysates (15 µg total protein) from U3A (negative control, Lane 1), Jurkat (Lane 2), and HeLa cells treated without (Lane 3) or with (Lane 4) 500 ng/mL nocodazole overnight were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-STAT1 clone A15158C (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301) for clone A15158C, and HRP donkey anti-rabbit IgG (Cat. No. 406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: MW ladder.
  • A15158C_Pure_Stat1_Antibody_1_082918
    Total cell lysates (15 µg total protein) from U3A (negative control, Lane 1), Jurkat (Lane 2), and HeLa cells treated without (Lane 3) or with (Lane 4) 500 ng/mL nocodazole overnight were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.25 µg/mL (1:2000 dilution) purified anti-STAT1 clone A15158C (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301) for clone A15158C, and HRP donkey anti-rabbit IgG (Cat. No. 406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: MW ladder.
  • A15158C_Pure_Stat1_Antibody_2_082918
    To confirm that the described nocodazole treatment worked, total cell lysates (15 µg total protein) from HeLa cells treated without (-) or with (+) nocodazole 500 ng/mL overnight were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) purified anti-STAT1 Serine 727 antibody (clone A15158B, left) or purified anti-STAT1 antibody (clone A15158C, right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301).
  • A15158C_Pure_Stat1_Antibody_3_042419.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.05 µg/mL (1:10000 dilution) of Purified anti-STAT1 Antibody, clone A15158C, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
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603701 25 µg £70
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603702 100 µg £174
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Description

STAT1, also known as signal transducter and activator of transcription 1, is a ubiquitously expressed protein that is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, cytosolic STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcriptional activator. Two isoforms of STAT1, with predicted molecular weights of 87 (STAT1α) and 83 kD (STAT1β), exist as a result of alternative RNA processing. This clone is specific for STAT1α. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT1 peptide phosphorylated at Serine 727.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.25 µg per ml (1:2000 - 1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for STAT1 isoform a and is not predicted to recognize the ß isoform due to the absence of the region corresponding to the immunizing peptide.

This clone displayed a stronger affinity for STAT1a in parallel western blot testing with BioLegend’s anti-STAT1 antibody (clone 10C4B40).

U3A cells were selected for antibody specificity testing because the cell line does not express STAT11.

This clone was derived from a human STAT1 peptide phosphorylated at Serine 727 immunogen, but tested negative for phospho-recognition while displaying strong pan-reactivity. Supplemental data is provided confirming the differences in phosphorylation of STAT1 Serine 727 in HeLa cells treated with or without nocodazole.

Application References

(PubMed link indicates BioLegend citation)
  1. Muller M, et al. 1993. EMBO J. 12:4221
Product Citations
  1. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
RRID
AB_2749867 (BioLegend Cat. No. 603701)
AB_2749867 (BioLegend Cat. No. 603702)

Antigen Details

Structure
STAT1α is a 750 amino acid protein with a predicted molecular weight of 87 kD; contains an SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Ubiquitously expressed; cytosolic and nuclear distribution

Function
Transcription activator/Cytokine Signaling
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Durbin JE, et al. 1996. Cell 8:443.
  2. Darnell JE Jr, et al. 1994. Science 264:1415.
  3. Chen X, et al. 1998. Cell 93:827.
  4. Ramana CV, et al. 2000. Oncogene 19:2619.
Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 on UniProt.org
Go To Top Version: 1    Revision Date: 04/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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