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Purified anti-STAT2 Antibody

Pricing & Availability
Clone
Poly6245 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly6245_PURE_STAT2_Antibody_031319
Total cell lysates (15 µg total protein) from U87-MG, HepG2, and NTERA-2 (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:500 dilution of purified anti-STAT2 antibody, clone Poly6245, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted STAT2 expression; predicted expression data was obtained from Human Protein Atlas.
  • 1_Poly6245_PURE_STAT2_Antibody_031319
    Total cell lysates (15 µg total protein) from U87-MG, HepG2, and NTERA-2 (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:500 dilution of purified anti-STAT2 antibody, clone Poly6245, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in descending of predicted STAT2 expression; predicted expression data was obtained from Human Protein Atlas.
  • 2_Poly6245_PURE_STAT2_Antibody_WB_062116
    Western blot analysis of HL60 (lane 1), HepG2 (lane 2), 293E (lane 3) and mouse brain (lane 4) using anti-STAT2 antibody (Poly6245). Purified anti-β-actin antibody (Poly6221) was used as loading control.
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624502 200 µL £201
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Description

STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT 2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT 3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT 1, p48, and CREB binding protein. The Poly6245 antibody recognizes the C-terminal region of human STAT2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide mapping to the carboxy terminus of human STAT2
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
Application

WB - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.


This product may contain other non-IgG subtypes.

Additional Product Notes

The 200 µL size can be used for approximately 20 Western blots.

RRID
SCR_001134 (BioLegend Cat. No. 624502)

Antigen Details

Structure
STAT family, contains coiled coil and SH2 domain, 113 kD
Distribution

Translocates into nucleus after phosphorylation. Expressed in skin, thymus.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT
Interaction
STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein
Modification
Phosphorylation (Tyr 690) by Janus kinase 1
Biology Area
Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Bluyssen HAR, et al. 1997. J. Biol. Chem. 272:4600.
2. Qureshi AA, et al. 1996. Mol. Cell. Biol. 16:288.

Regulation
Phosphorylated by signaling from IFN alpha receptor
Gene ID
6773 View all products for this Gene ID
UniProt
View information about STAT2 on UniProt.org
Go To Top Version: 5    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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