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Purified anti-STAT2 Phospho (Tyr690) Antibody

Pricing & Availability
Clone
W21151B (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 2, STAT113, Interferon alpha induced transcriptional activator, p113
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
W21151B_PURE_STAT2_Antibody_071423
Whole cell extracts (15 µg total protein) from serum starved A431 cells untreated (-) or treated (+) with IFN-α (10 ng/mL, 30 min) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-STAT2 phospho (Tyr690) (clone W21151B) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Equal STAT2 loading was confirmed by probing membranes with anti-pan STAT2 antibody. Lane M: Molecular weight marker
  • W21151B_PURE_STAT2_Antibody_071423
    Whole cell extracts (15 µg total protein) from serum starved A431 cells untreated (-) or treated (+) with IFN-α (10 ng/mL, 30 min) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-STAT2 phospho (Tyr690) (clone W21151B) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Equal STAT2 loading was confirmed by probing membranes with anti-pan STAT2 antibody. Lane M: Molecular weight marker
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619851 25 µg £93
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619852 100 µg £257
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Description

STAT2 is a member of the STAT family of transcription factors that is phosphorylated by receptor associated kinases in response to cytokines and growth factors. After activation, STAT2 forms heterodimers with STAT1. Once it is activated, STAT1 and STAT2 dimerize and are translocated to the nucleus to act as a transcription factor. STAT2 is an important mediator of type I interferon signaling; thus, after type-I IFN binds to the receptor, JAK kinases phosphorylate STAT2 and STAT1. This leads to their dimerization and, together with IRF9/ISGF3G, form the ISGF3 transcription factor. Type-I IFN /STAT2 signaling is important in innate immunity antiviral responses. Additionally, STAT2 is a regulator of mitochondrial fission by modulating phosphorylation of DNM1L. Diseases associated with STAT2 are the Immunodeficiency 44 and Pseudo-Torch Syndrome 3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human STAT2 phosphorylated at Tyr690
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068194 (BioLegend Cat. No. 619851)
AB_3068194 (BioLegend Cat. No. 619852)

Antigen Details

Structure
STAT2 is an 851 amino acids length protein with a predicted molecular weight of 97.9 kD. STAT2 contains a SH2 domain
Distribution

Cytoplasm/Nucleus

Function
Transcription factor, activation in depending of phosphorylation
Interaction
Interacts with IRF9/ISGF3G to form ISGF3 transcription factor complex
Biology Area
Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Innate Immune Signaling, Phospho-Proteins
Antigen References
  1. Ho J, et al. 2016. PLoS Biol. 14:e2000117.
  2. Fu X, et al. 1992. PNAS. 89:7840-3.
  3. Yu w, et al. 2020. Redox Biol. 37:101761
  4. Lee CJ, et al. 2020. Exp Mol Med. 52:1526-1536. 
Gene ID
6773 View all products for this Gene ID
UniProt
View information about STAT2 on UniProt.org
Go To Top Version: 1    Revision Date: 07/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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