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Purified anti-STAT4 Phospho (Tyr693) Antibody

Pricing & Availability
Clone
A19016A (See other available formats)
Regulatory Status
RUO
Other Names
Signal Transducer and Activator Of Transcription 4, SLEB11, Signal Transducer and Activator of Transcription 4 Variant 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-A19016A_PURE_STAT4_Phospho_Tyr693_Antibody_WB_031221
Whole cell extracts (15 µg protein) from serum-starved NK-92 cells untreated (-) or treated (+) with 100 ng/mL of recombinant human IL-2 (Cat. No. 589104) and 100 ng/mL of recombinant human IFN-α2 (Cat. No. 592702) for 20 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with 0.25 µg/mL (1:2000 dilution) purified anti-STAT4 Phospho (Tyr693) antibody (clone A19016A) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal STAT4 loading was confirmed using a pan STAT4 antibody. Lane M: Molecular weight marker.
  • a-A19016A_PURE_STAT4_Phospho_Tyr693_Antibody_WB_031221
    Whole cell extracts (15 µg protein) from serum-starved NK-92 cells untreated (-) or treated (+) with 100 ng/mL of recombinant human IL-2 (Cat. No. 589104) and 100 ng/mL of recombinant human IFN-α2 (Cat. No. 592702) for 20 minutes were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with 0.25 µg/mL (1:2000 dilution) purified anti-STAT4 Phospho (Tyr693) antibody (clone A19016A) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal STAT4 loading was confirmed using a pan STAT4 antibody. Lane M: Molecular weight marker.
  • b-A19016A_PURE_STAT4_Phospho_Tyr693_Antibody_ICFC_1_121322
    NK-92 cells untreated (negative control, open histogram), or treated with hIL-2 (100 ng/mL) and hIFNα2 (100 ng/mL) (positive control, solid histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with purified anti-Stat4 Phospho (Tyr693) (clone A19016A), or Mouse IgG1, κ isotype control (Cat No. 401401) (Open histogram, dashed line is representative for both, untreated and treated NK-92 cells) followed by PE-goat anti-mouse IgG (Cat No.405307).
  • c-A19016A_PURE_STAT4_Phospho_Tyr693_Antibody_ICFC_2_121322
    PBMC pre-treated overnight with cell activator cocktail (1:2500; Cat No. 423301), and then treated with (positive control, solid histogram) or without (negative control, open histogram) hIL-2 (100 ng/mL) and IFNα2 (100 ng/mL) for 30 min, fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Purified anti-Stat4 Phospho (Tyr693) (clone A19016A), or Mouse IgG1, κ isotype control (Cat No. 401401) (Open histogram, dashed line is representative for both, untreated and IL2/IFNα2 treated PBMC) followed by PE-goat-anti mouse IgG (Cat No. 405307).
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941201 25 µg £97
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941202 100 µg £226
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Description

The signal transducer and activator of transcription (STAT) family of transcription factors are DNA-binding proteins required for transcriptional regulation of several cellular and physiological processes in response to cytokine and growth factor stimulation. Inactive STAT proteins are present as cytosolic monomers. C-terminal tyrosine phosphorylation by upstream Janus kinases results in STAT dimerization, translocation to the nucleus, and increased affinity for response elements found in the regulatory promoter of STAT target genes.

In contrast to most other members of the STAT family, STAT4 expression is restricted to hematopoietic cells and responds to fewer cytokines, such as IL-12 and IL-2. STAT4, which requires phosphorylation at tyrosine 693 for activation, plays a pivotal role in the differentiation and proliferation of Th1 and Th17 cells, both of which are crucial effectors of chronic autoimmune disorders. In natural killer cells, STAT4 is required for normal development, maturation, and activation. Single nucleotide polymorphisms of STAT4 are implicated in the pathogenesis of systemic lupus erythematosis and rheumatoid arthritis, and are linked with increased risk of hepatocellular carcinoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is predicted to recognize mouse STAT4 phosphorylated at tyrosine 694 and rat STAT4 phosphorylated at tyrosine 693 due to complete sequence homology between the immunizing peptide and the respective murine STAT4 orthologs.

RRID
AB_2890857 (BioLegend Cat. No. 941201)
AB_2890857 (BioLegend Cat. No. 941202)

Antigen Details

Structure
STAT4 is a 748 amino acid protein with predicted molecular weight of 86 kD.
Distribution

Hematopoietic cells/Nucleocytoplasmic localization

Function
Transcription factor, cell signaling
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Antigen References

Trinchieri G, et al. 2003. Nat. Rev. Immunol. 3:133.
Remmers EF, et al. 2007. N. Engl J. Med. 357:977.
Mukasa R, et al. 2010. Immunity. 32:616
Rapp M, et al. 2017. Sci. Immunol. 2:3296.

Gene ID
6775 View all products for this Gene ID
UniProt
View information about STAT4 Phospho on UniProt.org
Go To Top Version: 2    Revision Date: 12/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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