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Purified anti-STAT6 Phospho (Tyr641) Antibody

Pricing & Availability
Clone
A15137A (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_A15137A_PURE_STAT6phospho_1_WB_Antibody_050318
Total cell lysate (15 µg protein) from non-treated and 100 ng/mL recombinant human IL-4 treated Daudi cells (10 min treatment) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) and 1:50000 (0.01 µg/mL) diluted purified anti-STAT6 phospho (Tyr641) antibody (clone A15137A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • 1_A15137A_PURE_STAT6phospho_1_WB_Antibody_050318
    Total cell lysate (15 µg protein) from non-treated and 100 ng/mL recombinant human IL-4 treated Daudi cells (10 min treatment) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) and 1:50000 (0.01 µg/mL) diluted purified anti-STAT6 phospho (Tyr641) antibody (clone A15137A) (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
  • 2_A15137A_PURE_STAT6phospho_2_IF_Antibody_081717
    Daudi cells treated with 100 ng/mL recombinant human IL-4 were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1 µg/ml anti-STAT6 phospho (Tyr641) antibody (clone A15137A) overnight at 4 degree followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG (clone Poly 4053) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 3_A15137A_PURE_STAT6phospho_3_IF_Antibody_081717
    Daudi cells un-treated were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1 µg/ml anti-STAT6 phospho (Tyr641) antibody (clone A15137A) overnight at 4 degree followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG (clone Poly 4053) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 4_A15137A_GoChIP_STAT6_Antibody_1_011518.PNG
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from Ramos cells starved overnight and then treated with IL-4 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT6 Phospho (Tyr641) (clone A15137A), or B) equal amount of Purified mouse Ig1, κ isotype control antibody (Cat. No. 401401), or C) competitor's ChIP-grade purified anti-STAT6 Phospho (Tyr641) antibody and D) equal amount of matched isotype control antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human DMD gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
Cat # Size Price Quantity Check Availability Save
690101 25 µg £97
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690102 100 µg £197
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT6 peptide phosphorylated at Tyr641.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunocytochemistry, a concentration range of 0.2 - 1.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms at 74, 82, and 94 kD. The predominant band detected is at 94 kD.

RRID
AB_2715760 (BioLegend Cat. No. 690101)
AB_2715760 (BioLegend Cat. No. 690102)

Antigen Details

Structure
847 amino acids, predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution

Translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Goenka S, et al. 2011. Immunol. Res. 50:87
  2. Wurster AL, et al. 2000. Oncogene. 19:2577
  3. Akira S, 1999. Stem Cells. 17:138
  4. Zamorano J, et al. 2005. J. Immunol. 174:2843
  5. David M, et al. 2001. Oncogene. 20:6660
  6. Takeda K, et al. 1996. Nature. 380:627
Gene ID
6778 View all products for this Gene ID
UniProt
View information about STAT6 Phospho Tyr641 on UniProt.org
Go To Top Version: 3    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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