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- Clone
- W21145C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MST1, KRS2, YSK3, MAmmalian STE20-Like protein kinase 1, Serine/Threonine-Protein Kinase Krs-2, Kinase responsive to stress 2
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with purified anti-STK4 (clone W21145C). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining with purified anti-STK4 (clone W21145C) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) purified anti-STK4 (clone W21145C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 673151 | 25 µg | £103 | ||||
| 673152 | 100 µg | £284 | ||||
Serine/Threonine Kinase 4, also known as STK4 or MST1, is essential for regulating cellular processes like stress response, apoptosis, and organ size control. Functionally, STK4 is a stress-activated kinase that can induce apoptosis by entering the nucleus and causing chromatin condensation and DNA fragmentation. It plays a crucial role in the Hippo signaling pathway, which restricts cell proliferation and promotes apoptosis, thereby preventing tumor growth. STK4 is regulated through its interactions with other proteins in a kinase cascade, including STK3/MST2 and LATS1/2, which further modulate proteins involved in cell growth and apoptosis. Mutations or deficiencies in STK4 can lead to severe immunodeficiencies and other health issues.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human STK4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use of this antibody on immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend using Tris-EDTA buffer (Cat. No. 422704) for antigen retrieval. We do not recommend citrate buffer.
Antigen Details
- Structure
- STK4 is a 487 amino acid protein with a molecular weight of approximately 55 kDa.
- Distribution
-
The STK4 protein is primarily localized in the cytoplasm but upon activation can be found in the nucleus
- Function
-
The regulation of STK4 involves several mechanisms:
- Phosphorylation: STK4 is activated through phosphorylation
- Protein Interactions: STK4 interacts with various proteins in the Hippo signaling pathway, including STK3/MST2 and LATS1/2
- Stress Response: STK4 is activated in response to cellular stress
- Negative Regulation: STK4 can negatively regulate certain pathways, such as the TBK1-IRF3 signaling pathway
- Interaction
- STK4 interacts with various proteins in the Hippo signaling pathway, including STK3/MST2 and LATS1/2.
- Antigen References
-
- Abdollahpour H, et al. 2012. Blood. 119:3450.
- Jorgensen SE, et al. 2020. J Clin Immunology. 41:109.
- Guennoun A, et al. 2021. J Clin Immunology. 41:1839.
- Nehme NT, et al. 2012. Blood. 119:3458.
- Gene ID
- 6789 View all products for this Gene ID
- UniProt
- View information about STK4 on UniProt.org
Related FAQs
Other Formats
View All STK4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-STK4 | W21145C | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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