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Purified anti-Sun2 Antibody

Pricing & Availability
Clone
3.1E (See other available formats)
Regulatory Status
RUO
Other Names
Sad1 and UNC84 domain containing 2, UNC84B,SUN Domain-Containing Protein 2, Rab5-Interacting Protein, FRIGG, Protein unc-84 homolog B, Rab5IP, Sad1/unc-84 protein-like 2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a. 31E_PURE_Sun2_WB_080223.
Whole cell extracts (15 µg total protein) from indicated cell lines or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-Sun2 (clone 3.1E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. 31E_PURE_Sun2_WB_080223.
    Whole cell extracts (15 µg total protein) from indicated cell lines or tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-Sun2 (clone 3.1E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. 31E_PURE_Sun2_ICC_080223
    NIH-3T3 cells were fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 µg/mL of purified anti-Sun2 (clone 3.1E), followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature (panels A and B). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A). The images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X water objective. Scale bar: 50 µm
  • c. 31E_PURE_Sun2_IHCP_080223
    IHC staining of purified anti-Sun2 (clone 3.1E) on formalin-fixed paraffin-embedded mouse brain. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated with 10 µg/mL of purified anti-Sun2 (clone 3.1E) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature (panels A and B). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A). Images were captured with a 40X objective. Scale bar: 50 µm
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620751 25 µg £134
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620752 100 µg £332
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Description

Sun2 is a transmembrane protein that localizes to the inner nuclear membrane and interacts with other nuclear proteins to form the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. This complex bridges the nuclear envelope, connecting the nuclear lamina to the cytoskeleton, thereby providing mechanical support and stability to the nucleus. Sun2 also plays a vital role in nuclear migration, chromosome organization, and gene regulation. Additionally, Sun2 is involved in maintaining nuclear envelope integrity during cell division and DNA repair, and has been suggested to act as a tumor suppressor by protecting against cancer-inducing DNA damage. In lung cancer, Sun2 has been shown to inhibit the Warburg effect via Glut1 and LDHA. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Residues 1-121 of human SUN2 fused to GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For IHC-P, antigen retrieval using Citrate buffer, pH 6.0 (Cat. No. 420901) is recommended.

For ICC, we recommend fixation/permeabilization with 100% ice-cold methanol for 10 minutes. Other fixation/permeabilization methods have not been tested.

RRID
AB_3097218 (BioLegend Cat. No. 620751)
AB_3097218 (BioLegend Cat. No. 620752)

Antigen Details

Structure
Sun2 is a 717 amino acid protein with a predicted molecular weight of 80.3 kD.
Distribution

Nuclear membrane, ubiquitously expressed in all cell types

Function
Structural protein
Interaction
Sun1, Nesprin 1g, Nesprin 1α
Biology Area
Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family
Nuclear Markers
Antigen References
  1. Stroud M, et al. 2014. Circ. Res. 114538-48.
  2. Chen X, et al. 2019. Oncol Lett. 17:01-1408.
  3. Meinke P, et al.2011. Biochem Soc Trans. 39:1693-7.
  4. Lv X, et al. 2016. Sci Rep. 5:17940. 
Gene ID
25777 View all products for this Gene ID
UniProt
View information about Sun2 on UniProt.org
Go To Top Version: 1    Revision Date: 08/02/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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