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Purified anti-Syk Antibody

Pricing & Availability
Clone
SYK-01 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
syk_112907
Total cell lysates (15 µg total protein) from A549 (negative control), HeLa (negative control), HEL, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Syk antibody, clone SYK-01, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • syk_112907
    Total cell lysates (15 µg total protein) from A549 (negative control), HeLa (negative control), HEL, Ramos, and A20 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:5000 dilution) of purified anti-Syk antibody, clone SYK-01, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • SYK-01_IHC_062508
    Formalin-fixed paraffin-embedded human tonsil tissue was stained with SYK-01 at 10 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
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626201 25 µg £81
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626202 100 µg £201
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Description

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites. The SYK-01 monoclonal antibody (raised against amino acids 5-360 of human Syk) recognizes human, mouse, and rat and Syk and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant (amino acids 5-360) human Syk
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml. For IHC, use a 10 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

This clone may produce a non-specific band of roughly ~250 kD at higher antibody concentrations

This clone does not recognize ZAP70

This clone has been reported to recognize rat Syk

Application References

(PubMed link indicates BioLegend citation)
  1. Arudchandran R, et al. 2000. J. Exp. Med. 191:47. (IP WB)
  2. Swann PG, et al. 1999. J. Biol. Chem. 33:23068. (IP)
  3. Inoue M, et al. 2011. J. Immunol. 186:18. PubMed
  4. Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed
  5. Cunningham CC, et al. 2012. Clin Immunol. 144:228. PubMed
  6. Alsadeg A, et al. 2014. J Immunol. 193:268. PubMed
Product Citations
  1. Duque-Afonso J, Lin CH 2016. Cancer Res . 76(23):6937-6949. PubMed
  2. Hatterer E, et al. 2011. Neurosci Res. 70:172. PubMed
  3. Cunningham C, et al. 2012. Clin Immunol. 144:228. PubMed
  4. Zheng S, et al. 2019. PLoS Biol. 17:e3000569. PubMed
  5. Hüttl S, et al. 2015. J Immunol. 195: 1548-1563. PubMed
  6. Inoue M, et al. 2011. J Immunol. 186:19:00. PubMed
  7. Alsadeq A, et al. 2014. J Immunol. 193:268. PubMed
RRID
AB_2200268 (BioLegend Cat. No. 626201)
AB_2200268 (BioLegend Cat. No. 626202)

Antigen Details

Structure
Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution

Cytoplasmic protein, widely expressed in hematopoietic cells

Function
Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Interaction
c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, paxillin, FAK, Grb2, CD3ε, LAT, many others
Modification
Phosphorylation (Y131, Y323, Y348, Y352, Y525, Y526)
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790.
2. Toyabe S-L, et al. 2001.Immunology 103:164.

Gene ID
6850 View all products for this Gene ID
UniProt
View information about Syk on UniProt.org
Go To Top Version: 3    Revision Date: 07/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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