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Purified anti-Tau, 269-281 Recombinant Antibody

Pricing & Availability
Clone
QA18A48 (See other available formats)
Regulatory Status
RUO
Other Names
TAU, MSTD, PPND, DDPAC, MAPT, MTBT1, MTBT2, tau-40, FTDP-17, PPP1R103
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_QA18A48_PURE_Tau269-281_Recom_Antibody_1_022522
Total tissue lysates (15 µg total protein) from human brain (positive control, Lane 1) and mouse brain (positive control, Lane 2) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL (1:500) anti-Tau, 269-281 recombinant (clone QA18A48) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight ladder
  • 1_QA18A48_PURE_Tau269-281_Recom_Antibody_1_022522
    Total tissue lysates (15 µg total protein) from human brain (positive control, Lane 1) and mouse brain (positive control, Lane 2) were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 μg/mL (1:500) anti-Tau, 269-281 recombinant (clone QA18A48) overnight at 4 °C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight ladder
  • 2_QA18A48_PURE_Tau269-281_Recom_Antibody_2_022522
    IHC staining of anti-Tau, 269-281 recombinant (clone QA18A48) on formalin-fixed paraffin-embedded human brain (A, positive control) and liver (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of recombinant anti-Tau, 269-281 overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm
  • 3_QA18A48_PURE_Tau269-281_Recom_Antibody_3_022522
    Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 0N3R (blue) and 0N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system.
  • 4_QA18A48_PURE_Tau269-281_Recom_Antibody_4_022522
    Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 1N3R (blue) and 1N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system.
  • 5_QA18A48_PURE_Tau269-281_Recom_Antibody_5_022522
    Direct ELISA of anti-Tau, 269-281 recombinant (clone QA18A48) binding to plate-immobilized recombinant human 2N3R (blue) and 2N4R (red) Tau proteins. ELISA was performed by coating wells with 1 µg/mL of Tau proteins. The wells were then incubated with the primary antibody at room temperature for 2 hours, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (3, 3', 5, 5' tetramethylbenzidine) (Cat. No. 421501) Substrate solution was used as the detection system.
Cat # Size Price Quantity Check Availability Save
948801 25 µg £89
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948802 100 µg £221
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
Partial recombinant human Tau protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1 - 25 µg/mL is suggested. For Direct ELISA, the suggested use of this reagent is 0.2 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous human and mouse Tau proteins in brain lysates by WB and reacts with 0N3R, 0N4R, 1N3R, 1N4R, 2N3R, and 2N4R recombinant Tau proteins by direct ELISA.

RRID
AB_2910513 (BioLegend Cat. No. 948801)
AB_2910513 (BioLegend Cat. No. 948802)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References
  1. Yipen W, et al. 2016. Nat Rev Neurosci 17:5-21.
  2. Dong Y, et al. 2012. PLoS One 7:e39386.
  3. Augustinack JC, et al. 2002. Acta Neuropathol. 103:26-35. 
  4. Seubert P, et al. 1995. J Biol Chem 270:1817-22.
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau 269-281 on UniProt.org
Go To Top Version: 1    Revision Date: 02/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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