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Purified anti-TAZ (WWTR1) Antibody

Pricing & Availability
Clone
W22256A (See other available formats)
Regulatory Status
RUO
Other Names
Transcriptional coactivator with PDZ-binding motif (TAZ), WW domain-containing transription regulator protein 1 (WWTR1)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A - W22256A_Purified_anti-TAZ_WWTR1_Antibody_4_091924
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-TAZ (WWTR1) (clone W22256A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • A - W22256A_Purified_anti-TAZ_WWTR1_Antibody_4_091924
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-TAZ (WWTR1) (clone W22256A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • B - W22256A_Purified_anti-TAZ_WWTR1_Antibody_1_091924
    IHC staining of Purified anti-TAZ (WWTR1) (clone W22256A) on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated either without (panel A) or with (panel B) Purified anti-TAZ (WWTR1) (clone W22256A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
  • C - W22256A_Purified_anti-TAZ_WWTR1_Antibody_2_091924
    U251-MG cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton-X for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were either stained with Purified Rat IgG2a, κ Isotype Control (Cat No. 400502) (panel A) or Purified anti-TAZ (WWTR1) (clone W22256A) (panel B), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 μm
  • D - W22256A_Purified_anti-TAZ_WWTR1_Antibody_3_091924
    U251-MG cells (positive cell line, filled histogram) and SH-SY5Y cells (negative cell line, open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat No. 425401) and intracellularly stained with Purified anti-TAZ (WWTR1) (clone W22256A), or Purified Rat IgG2a, κ Isotype Control (open histogram, dashed line) (Cat No. 400502).
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673551 25 µg £117
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673552 100 µg £295
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Description

TAZ, also known as WWTR1, is a critical regulator in various cellular processes including cell proliferation, differentiation, and organ development. It is a key component of the Hippo signaling pathway, which controls organ size by regulating cell growth and apoptosis. TAZ functions primarily as a transcriptional co-activator, partnering with transcription factors such as TEAD to promote the expression of genes involved in cell cycle progression and survival. Additionally, it plays a role in maintaining stem cell pluripotency and promoting epithelial-mesenchymal transition (EMT), a process important in development and cancer metastasis. Aberrations in TAZ activity are linked to various cancers, highlighting its significance in both normal physiology and disease pathology. TAZ should not be confused with tafazzin, which was previously also known as Taz/Taz1. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the N-terminus of human TAZ (WWTR1)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

ICC, ICFC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone W22256A) in immunocytochemistry (ICC) we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with either 0.5% Triton-X or 100% ice-cold methanol. Permeabilization with 100% ice-cold methanol results in a dimmer signal compared to 0.5% Triton-X. Fixation/permeabilization with 100% ice-cold methanol alone is not recommended. 

For use of this antibody (clone W23013A) in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using fixation buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401).  

For use of this antibody (clone W23013A) in immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422703). Antigen retrieval with Sodium Citrate H.I.E.R. (Cat. No. 928502) is not recommended.

Antigen Details

Structure
TAZ is a 400 amino acid protein with a predicted molecular weight of 44.1 kD.
Distribution

Predominantly nucleus, with diffuse localization in the cytoplasm at high expression levels

Function
Transcription factor, transcriptional co-activator
Interaction
TEA domain family members (TEAD1/2/3/4), PAX3, RUNX1/2, YAP1, AP1, SMAD2/3/4
Biology Area
Cell Biology, Cell Proliferation and Viability, Stem Cells, Transcription Factors
Antigen References
  1. Piccolo S, et al. 2014. Physiol. Rev. 94:1287-312. 
  2. Moya I, Halder G. 2019. Nat. Rev. Mol. Cell Biol. 20:211-226.
  3. Pocaterra A, et al. 2020. J. Cell Sci. 133:jcs230425. 
  4. Wei Y, et al. 2023. MedComm. 4:e340.
Gene ID
25937 View all products for this Gene ID
UniProt
View information about TAZ on UniProt.org
Go To Top Version: 1    Revision Date: 09/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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