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Purified anti-TCF12 Antibody

Pricing & Availability
Clone
W16168A (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor 12, Class B basic helix-loop-helix protein 20 (bHLHb20), HEB, HTF4
Isotype
Rat IgG2b, κ
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Product Citations
publications
W16168A_PURE_TCF12_Antibody_061417
Total cell lysate (15µg protein) from MCF-7 (negative control), Jurkat and EL4 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TCF12 antibody (clone W16168A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody for clone W16168A or a 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • W16168A_PURE_TCF12_Antibody_061417
    Total cell lysate (15µg protein) from MCF-7 (negative control), Jurkat and EL4 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TCF12 antibody (clone W16168A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody for clone W16168A or a 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
698701 25 µg £70
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698702 100 µg £174
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Description

TCF12, also known as HTF4 or HEB, is a member of class I basic helix-loop-helix (bHLH) protein family that recognizes the consensus E-box (5'-CANNTG-3') DNA binding site. TCF12 is a transcription factor that participates in regulating gene expression through formation of homodimer or heterodimers with other bHLH E-proteins. TCF12 has been shown to be involved in neuron differentiation and is upregulated in certain cancer cells. TCF12 functions as a transcriptional repressor responsible for down-regulating expression of E-cadherin in colorectal cancer cells. TCF12-deficient mice show abnormality in brain development and a high percentage of postnatal death. Mutations in TCF12 gene have been linked to coronal craniosynostosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human TCF12 recombinant protein (108-521 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 diluted). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687319 (BioLegend Cat. No. 698701)
AB_2687319 (BioLegend Cat. No. 698702)

Antigen Details

Structure
706 amino acids with a predicted molecular weight of 76 kD. Contains a C-terminal bHLH domain responsible for DNA binding.
Distribution

Nucleus

Function
TCF12 is a transcriptional regulator involved in neuronal development and is up-regulated in some type of tumors.
Interaction
Forms a homodimer or a heterodimer with myogenin, E12 and ITF2 proteins. Interacts with PTF1A, NEUROD2, RUNX1T1, AML1-MTG8/ETO, and BHLHA9.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yi S, et al. 2017. Stem Cells. 35:386.
2. Chen YF, et al. 2016. Cancer Res. 76:4872.
3. Goos JA, et al. 2016. Hum. Mutat. 37:732.
4. Labreche K, et al. 2015. Nat. Commun. 6:7207.
5. Sharma VP, et al. 2013. Nat. Genet. 45:304.
6. Lee CC, et al. 2012. J. Biol. Chem. 287:2798.

Gene ID
6938 View all products for this Gene ID
UniProt
View information about TCF12 on UniProt.org
Go To Top Version: 0    Revision Date: 06/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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