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Purified anti-TCP-1β Antibody

Pricing & Availability
Clone
F39P7F11 (See other available formats)
Regulatory Status
RUO
Other Names
CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_F39P7F11_Pure_TCP-1B_Antibody_113018
Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_F39P7F11_Pure_TCP-1B_Antibody_113018
    Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_TCP-1beta_F39P7F11_111010
    HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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635502 100 µg £157
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Description

TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kD, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Frog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide RKRVPDHHPC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
Product Citations
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
  2. Arines FM, et al. 2021. J Cell Biol. 220:. PubMed
RRID
AB_2275598 (BioLegend Cat. No. 635502)

Antigen Details

Structure
Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution

Cytoplasm

Function
In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
Interaction
β-actin, 14-3-3γ, SUMO4, TCP-1α, β-Tubulin, α-tubulin, CCT8
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References

1. Yaffe MB, et al. 1992. Nature 358:245

Gene ID
21454 View all products for this Gene ID
UniProt
View information about TCP-1 beta on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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