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Purified anti-TdT Antibody

Pricing & Availability
Clone
7H9A33 (See other available formats)
Regulatory Status
RUO
Other Names
DNA nucleotidylexotransferase (DNTT), Terminal addition enzyme, Terminal transferase
Isotype
Mouse IgG1, κ
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Product Citations
publications
7H9A33_TdT_Purified_Antibody_1_031919
Whole cell extracts (15 µg protein) from Daudi (negative control) and Jurkat cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-TdT Antibody, clone 7H9A33, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted DNTT expression obtained from Human Protein Atlas.
  • 7H9A33_TdT_Purified_Antibody_1_031919
    Whole cell extracts (15 µg protein) from Daudi (negative control) and Jurkat cells were resolved on a 4-20% Tris-glycine gel, transferred to a nitrocellulose membrane and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-TdT Antibody, clone 7H9A33, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted DNTT expression obtained from Human Protein Atlas.
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659002 100 µg £201
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Description

Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TdT recombinant protein (1-508aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 7H9A33 clone has been shown to be useful for western blotting of human and mouse TdT.

RRID
AB_2562962 (BioLegend Cat. No. 659002)

Antigen Details

Structure
510 amino acids, predicted molecular weight of 58 kD.
Distribution

Nucleus.

Function
Template-independent DNA polymerase catalyzing the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. It is also involved in the addition of nucleotides at the N region junction of rearranged immunoglobulin heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Interaction
Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone.
Cell Type
B cells
Biology Area
Cell Biology, DNA Repair/Replication, Immunology
Molecular Family
Enzymes and Regulators
Antigen References

1. Yunk L, et al. 2009. J. Immunol. 183:452.
2. Kedzierska K, et al. 2008. J. Immunol. 181:2556.
3. Fuller SA, et al. 1985. Biochem. J. 231:105.
4. Feeney AJ, et al. 2001. J. Immunol. 167:3486.
5. Komori T, et al. 1993. Science 261:1171.

Gene ID
1791 View all products for this Gene ID
UniProt
View information about TdT on UniProt.org
Go To Top Version: 5    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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