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Purified anti-TFAM Antibody

Pricing & Availability
Clone
18G102B2E11 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor A, mitochondrial, mtTFA, MtTF1, TCF6, TCF-6, Transcription Factor 6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_18G102B2E11_PURE_TFAM_Antibody_WB_083117
Western blots of anti-TFAM antibody (clone 18G102B2E11) and isotype-matched control mouse IgG2b. Lane 1: 20 µg of normal human brain lysate; Lane 2: 20 µg of human brain membrane lysate. Blots were incubated overnight at 4°C with 1 or 5 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • 1_18G102B2E11_PURE_TFAM_Antibody_WB_083117
    Western blots of anti-TFAM antibody (clone 18G102B2E11) and isotype-matched control mouse IgG2b. Lane 1: 20 µg of normal human brain lysate; Lane 2: 20 µg of human brain membrane lysate. Blots were incubated overnight at 4°C with 1 or 5 µg/ml of primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse IgG secondary antibody. Enhanced Chemiluminescence was used as the detection system.
  • 2_18G102B2E11_PURE_TFAM_Antibody_IHC_1_083117
    IHC staining of anti-TFAM antibody (clone 18G102B2E11) and isotype-matched control mouse IgG2b on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using sodium citrate and 0.25% triton X-100, the tissue was incubated with primary antibodies at 5 µg/ml overnight at 4°C. Tissue-bound antibody was detected using BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit and DAB (3,3'-diaminobenzidine) substrate.
  • 3_18G102B2E11_PURE_TFAM_Antibody_IHC_2_083113
Cat # Size Price Quantity Check Availability Save
850501 25 µg £94
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850502 100 µg £230
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Description

TFAM (Transcription Factor A, Mitochondrial) binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation, and DNA replication and repair. The protein is required for accurate and efficient promoter recognition by mitochondrial RNA polymerase. Sequence polymorphisms in the TFAM gene are associated with Alzheimer's and Parkinson's diseases. Other diseases associated with TFAM include Mitochondrial DNA Depletion Syndrome, Hepatocerebral Form and Deoxyguanosine Kinase Deficiency.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human TFAM protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes endogenous TFAM in human brain tissues in WB and IHC applications but does not recognize mouse TFAM.

Application References

(PubMed link indicates BioLegend citation)
  1. Wang YE, et al. 2013. PLoS One 8(8):e74513. (WB)
  2. Caielli S, et al. 2016. J Exp Med. 213(5):697-713. (WB)
Product Citations
  1. Fihurka O, et al. 2023. Biomolecules. 13:. PubMed
  2. von Walden F, et al. 2021. Dev Med Child Neurol. 63:1204. PubMed
  3. Fihurka O, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2716009 (BioLegend Cat. No. 850501)
AB_2716009 (BioLegend Cat. No. 850502)

Antigen Details

Structure
TFAM is synthesized as a precursor form with a mitochondrial targeting peptide, which is removed by processing after import to the mitochondria. Mature TFAM has an apparent molecular weight of ~25 kD. Alternative splicing results in multiple transcript variants.
Distribution

Tissue distribution: Central nervous system, skin, kidney, lung, thyroid, lymph nodes, breast, esophagus, and heart.

Cellular distribution: Mitochondrion, nucleus, and cytosol.

Function
TFAM functions in mitochondrial transcription regulation, and DNA replication and repair.
Interaction
TFB1M, TFB2M, CLPX
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Mitochondrial Markers
Antigen References

1. Bestwick ML, Shadel GS. 2013. Trends Biochem Sci 38(6):283-91.
2. Ramachandran A, et al. 2017. Nucleic Acids Res 45(2):861-874.
3. Günther C, et al. 2004. Neurosci. Lett. 369: 219–223.

Gene ID
7019 View all products for this Gene ID
UniProt
View information about TFAM on UniProt.org

Other Formats

View All TFAM Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-TFAM 18G102B2E11 WB,IHC-P
Biotin anti-TFAM 18G102B2E11 WB
Go To Top Version: 0    Revision Date: 08/31/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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