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Purified anti-TGF-β1 Antibody

Pricing & Availability
Clone
O92B5 (See other available formats)
Regulatory Status
RUO
Other Names
TGF-Beta-1, TGFB, Latency-Associated Peptide, DPD1, LAP, CED
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
O92B5_PURE_TGF-beta1_Antibody_1_WB_082416
Western blot of anti-TGF-β1 antibody (clone O92B5). Lane 1: Molecular weight marker; Lane 2: 30 µg of human spleen lysate; Lane 3: 30 µg of human skin lysate; Lane 4: 20 µg of A549 cell lysate; Lane 5: 20 µg of U251 cell lysate. The blot was incubated with the primary antibody at 10 µg/ml overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody.
  • O92B5_PURE_TGF-beta1_Antibody_1_WB_082416
    Western blot of anti-TGF-β1 antibody (clone O92B5). Lane 1: Molecular weight marker; Lane 2: 30 µg of human spleen lysate; Lane 3: 30 µg of human skin lysate; Lane 4: 20 µg of A549 cell lysate; Lane 5: 20 µg of U251 cell lysate. The blot was incubated with the primary antibody at 10 µg/ml overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody.
  • O92B5_PURE_TGF-beta1_Antibody_2_IHCP_082416
    IHC staining of anti-TGF-β1 antibody (clone O92B5) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody for two hours at room temperature at 5 µg/ml. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by counterstaining with hematoxylin.
See high resolution IHC-P data...
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846802 100 µg £238
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Description

TGF-β1 is a member of the transforming growth factor beta superfamily of cytokines. TGF-β1 is produced as a large protein precursor, containing 390 amino acids, that is proteolytically processed to produce a mature peptide of 112 amino acids. It is a secreted protein that has different effect on different cell types or developmental stages some of which include immune cell regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human TGF-β1 recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 5.0 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629662 (BioLegend Cat. No. 846802)

Antigen Details

Structure
TGF-β1 is a 390 amino acid protein with a molecular mass of 44 kD.
Distribution

Tissue distribution:bone, blood, and epithelial cells.
Cellular distribution: extracellular, golgi apparatus, nucleus, plasma membrane, and cytoskeleton.

Function
TGF-β1 is a protein involved in the regulation of many cellular processes such as cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, tissue remodeling, wound healing, and stimulates ECM deposition and angiogenesis.
Interaction
Multiple cells express the TGF-β1 receptors.
Ligand/Receptor
TGF-β1 receptors.
Cell Type
Epithelial cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Diniz LP, et al. 2014. Neurochem. 78:18. PubMed
2. Martinez-Canabal A. 2015. Int. J. Neurosci. 125:1. PubMed
3. Gomes FC, et al. 2005. Int. J. Dev. Neurosci. 23:413. PubMed
4. Krieglstein K, et al. 2002. J. Physiol. Paris 1-2:25. PubMed

Gene ID
7040 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org
Go To Top Version: 1    Revision Date: 08/24/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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