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Purified anti-Thrombospondin 1 Antibody

Pricing & Availability
Clone
W20027B (See other available formats)
Regulatory Status
RUO
Other Names
Glycoprotein G, THBS-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20027B_PURE_Thrombospondin-1_Antibody_020122
Whole cell extracts (15 µg total protein) were prepared from Daudi, NTERA-2, HeLa, and HUVEC cells. Proteins were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-THBS1 (clone W20027B) at 0.5 µg/mL (1:1000 dilution) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin (Cat. No. 643808) was used as a loading control at a 1:2000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W20027B_PURE_Thrombospondin-1_Antibody_020122
    Whole cell extracts (15 µg total protein) were prepared from Daudi, NTERA-2, HeLa, and HUVEC cells. Proteins were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-THBS1 (clone W20027B) at 0.5 µg/mL (1:1000 dilution) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin (Cat. No. 643808) was used as a loading control at a 1:2000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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600951 25 µg £81
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600952 100 µg £201
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Description

Thrombospondin 1 (THBS1) is a member of the thrombospondin family, a group of matricellular proteins that comprise a part of the extracellular matrix. Originally discovered in platelets, THBS1 mediates cell-cell interactions interacting with numerous ligands including heparin, integrins, CD36, and CD47. Through this activity, THBS1 regulates cell adhesion, motility and growth. THBS1 has been implicated in the activity of various cancers, by mediating angiogenesis and tumor cell invasion, and also plays a role in heart disease, fibrosis, and eye health. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant N-terminal fragment of THBS1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910473 (BioLegend Cat. No. 600951)
AB_2910473 (BioLegend Cat. No. 600952)

Antigen Details

Structure
THBS1 is a 1,170 amino acid protein with a predicted molecular weight of 130 kD.
Distribution

Secreted protein expressed by multiple cell types including platelets, smooth muscle cells, fibroblasts, and granulocytes

Function
TGF-β signaling, angiogenesis
Ligand/Receptor
Heparin, HSPG, sulfatides, versican, calreticulin, integrins, MMPs, TGF-β, CD36, CD47, CD148
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Motility/Cytoskeleton/Structure
Molecular Family
Adhesion Molecules
Antigen References
  1. Zhang K, et al. 2020. Int J Mol Med. 45:1275-1293.
  2. Wang P, et al. 2020. Curr Pharm Des. 26:2116-2136. 
Gene ID
7057 View all products for this Gene ID
UniProt
View information about Thrombospondin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/01/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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