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Purified anti-TIF1β (KAP-1, TRIM28) Antibody

Pricing & Availability
Clone
20A1 (See other available formats)
Regulatory Status
RUO
Other Names
Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
20A1_PURE_TIF1b_Antibody_1_WB_072216
Whole cell lysates (15 µg protein) from HeLa, HEK293, and RAW264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-TIF1β (KAP-1, TRIM28) antibody, clone 20A1. Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse IgG secondary antibody (1:3000 dilution, Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TRIM28 mRNA expression levels; HEK293 cells express ~8-fold more TRIM28 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker.
  • 20A1_PURE_TIF1b_Antibody_1_WB_072216
    Whole cell lysates (15 µg protein) from HeLa, HEK293, and RAW264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-TIF1β (KAP-1, TRIM28) antibody, clone 20A1. Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse IgG secondary antibody (1:3000 dilution, Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH antibody (1:2000 dilution, Cat. No. 649203) was used as a loading control (lower). Lane M: MW ladder. Cell lysates were loaded in order of increasing TRIM28 mRNA expression levels; HEK293 cells express ~8-fold more TRIM28 mRNA than HeLa cells (source: Human Protein Atlas). Lane M: Molecular Weight marker.
  • 20A1_PURE_TIF1b_Antibody_2_IF_022514
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-TIF1β (KAP-1, TRIM28) (clone 20A1) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
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619301 25 µg £70
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619302 100 µg £150
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Description

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
ChIP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg/mL.

Application References

(PubMed link indicates BioLegend citation)
  1. Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)
Product Citations
  1. Yang DM, et al. 2024. Nat Commun. 780:15. PubMed
  2. Ouyang C, et al. 2022. Int J Biol Sci. 18:2655. PubMed
RRID
AB_2209724 (BioLegend Cat. No. 619301)
AB_2209724 (BioLegend Cat. No. 619302)

Antigen Details

Structure
Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD
Distribution

Nuclear, associates with specific chromatin regions

Function
Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

 

Gene ID
10155 View all products for this Gene ID
UniProt
View information about TIF1beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 05/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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