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Purified anti-TIF1γ/TRIM33 Antibody

Pricing & Availability
Clone
3H7-C8-B9 (See other available formats)
Regulatory Status
RUO
Other Names
Tripartite Motif Containing 33,TIF1G,RFG7.TIFGAMMA,TF1G,PTC7,E3 Ubiquitin-Protein Ligase,RING-Type E3 Ubiquitin Transferase,RET-Fused Gene 7,KIAA1113,Ectodermin,Transcription intermediary factor 1-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3H7-C8-B9_PURE_TIF1gamma-TRIM33_Antibody_020623
Whole cell extracts (15 µg total protein) of the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL purified anti-TIF1γ/TRIM33 (clone 3H7-C8-B9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • 3H7-C8-B9_PURE_TIF1gamma-TRIM33_Antibody_020623
    Whole cell extracts (15 µg total protein) of the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL purified anti-TIF1γ/TRIM33 (clone 3H7-C8-B9) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
  • 3H7-C8-B9_PURE_TIF1gamma-TRIM33_Antibody_2_020623
    ICC staining of purified anti-TIF1γ/TRIM33 (clone 3H7-C8-B9) on MCF7 cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 2.5 µg/mL of anti-TIF1γ/TRIM33 (clone 3H7-C8-B9) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) (panel A) for 1 hour at room temperature. Nuclei were counterstained with DAPI (panel B) (Cat. No. 422801), and images were captured with 60X objective. Overlay image (panel C). Scale bar: 10 µm
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611251 25 µg £93
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611252 100 µg £260
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Description

TIF1γ/TRIM33, belonging to the tripartite motif-containing (TIF) protein family, is a multifunctional protein that regulates hematopoiesis, DNA repair, mitosis, and transcription elongation. Just as with other TIF/TRIM family members, TIF1γ/TRIM33 contains a conservative RBCC/TRIM motif at the N terminus and a PHD/ bromodomain at the C terminus.  TIF1γ/TRIM33 suppresses the TGF-beta/BMP signaling pathway via monoubiquitylation of SMAD4 or the binding of phosphorylated SMAD2/3. Through its E3 ubiquitin ligase function, TIF1γ/TRIM33 decreases nuclear beta-catenin expression and suppresses tumorigenesis and tumor cell proliferation. TIF1γ/TRIM33’s tumor suppressive properties have been observed in hepatocellular carcinoma, chronic myelomonocytic leukemia, and pancreatic cancer. It has also been observed to reduce the stability of HIV-1 viral integrase to restrict HIV-1 infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli derived recombinant fragment of human TIF1γ/TRIM33
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product is not recommended for use in IHC-P. 

For immunocytochemistry staining, the suggested fixation method is 4% paraformaldehyde.

RRID
AB_2936736 (BioLegend Cat. No. 611251)
AB_2936736 (BioLegend Cat. No. 611252)

Antigen Details

Structure
TIF1γ/TRIM33 is a 1127 amino acid protein with an expected molecular weight of 123 kD. It contains a RING domain, two B-box domains, and a coiled-coil domain (RBCC) at the N terminus; a plant homeodomain (PHD) and a bromodomain at the C terminus.
Distribution

Ubiquitous/ Mainly nuclear, some cytosolic

Function
Acts as E3 ubiquitin ligase to ubiquitylate target proteins, transcription inhibitor of TGF-beta/BMP pathway
Interaction
Homooligomer and heterooligomer with TRIM24 and TRIM28; interacts with SMAD2, SMAD3, and SMAD4.
Biology Area
Cell Cycle/DNA Replication, DNA Repair/Replication, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Enzymes and Regulators, Ligases, Tumor Suppressors
Antigen References
  1. Xue J, et al. 2015. Nat Commun. 6:6156.
  2. Herquel B, et al. 2011. Proc Natl Acad Sci U. S. A. 108:8212-7.
  3. Ali H, et al. 2019. Nat Commun. 10:926.
Gene ID
51592 View all products for this Gene ID
UniProt
View information about TIF1gamma/TRIM33 on UniProt.org
Go To Top Version: 1    Revision Date: 02/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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