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Purified anti-TIGAR Antibody

Pricing & Availability
Clone
W18015A (See other available formats)
Regulatory Status
RUO
Other Names
TP53 Induced Glycolysis Regulatory Phosphatase, TP53-Induced Glycolysis And Apoptosis Regulator, Fructose-2,6-Bisphosphatase TIGAR, C12orf5, Fructose-2,6-Bisphosphate 2-Phosphatase, Chromosome 12 Open Reading Frame 5, Transactivated By NS3TP2 Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18015A_PURE_TIGAR_Antibody_1_021820
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-TIGAR antibody (clone W18015A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in ascending order of predicted TIGAR expression; predicted expression data was obtained from Human Protein Atlas.
  • W18015A_PURE_TIGAR_Antibody_1_021820
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-TIGAR antibody (clone W18015A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in ascending order of predicted TIGAR expression; predicted expression data was obtained from Human Protein Atlas.
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935401 25 µg £70
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935402 100 µg £174
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Description

Tp53-induced glycolysis and apoptosis regulator (TIGAR) is a negative regulator of glycolysis upregulated by p53 in response to cellular stresses and it protects cells against reactive oxygen species (ROS)-induced autophagy. TIGAR decreases the rate of glycolysis by reducing the level of fructose-2, 6-bisphosphate, which is an activator of phosphofructokinase-1 (PFK1), a rate-limiting enzyme in glycolysis. Additionally, TIGAR promotes the pentose phosphate pathway and provides NADPH for glutathione reductase to generate reduced glutathione (GSH). GSH lowers the intracellular level of ROS and prevents DNA damage, thereby promoting cell survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human TIGAR protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse TIGAR.

The clone produced incorrect staining in ICC testing.

The clone could not pull down TIGAR in IP testing.

RRID
AB_2832894 (BioLegend Cat. No. 935401)
AB_2832894 (BioLegend Cat. No. 935402)

Antigen Details

Structure
TIGAR is a 270 amino acid protein with a predicted molecular weight of 30 kD.
Function
Oxidative stress, cancer metabolism
Biology Area
Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References
  1. Green D, et al. 2006. Cell. 126:30.
  2. Bensaad K, et al. 2006. Cell. 126:107.
  3. Cheung E, et al. 2012. Proc Natl Acad Sci USA. 109:20491.
  4. Wanka C, et al. 2012. J. Biol. Chem. 287:33436.
Gene ID
57103 View all products for this Gene ID
UniProt
View information about TIGAR on UniProt.org
Go To Top Version: 1    Revision Date: 02/18/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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