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Purified anti-TOX Antibody

Pricing & Availability
Clone
6E6D03 (See other available formats)
Regulatory Status
RUO
Other Names
Thymocyte Selection-Associated High Mobility Group Box, Thymus High Mobility Group Box Protein TOX, TOX1, KIAA0808
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6E6D03_PURE_TOX_Antibody_1_WB_071018
Total cell lysates (15 µg protein) from Jurkat (lane 1), Molt-4 (lane 2), THP-1 (lane 3), HeLa (lane 4) and NIH3T3 (lane 5) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of Purified anti-TOX Antibody (clone 6E6D03) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TOX Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 6E6D03_PURE_TOX_Antibody_1_WB_071018
    Total cell lysates (15 µg protein) from Jurkat (lane 1), Molt-4 (lane 2), THP-1 (lane 3), HeLa (lane 4) and NIH3T3 (lane 5) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of Purified anti-TOX Antibody (clone 6E6D03) (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-mouse-IgG secondary antibody conjugated to HRP for anti-TOX Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 6E6D03_PURE_TOX_Antibody_2_ICC_071018
    Jurkat cells were stained with purified anti-TOX (clone 6E6D03) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Nuclei were stained with DAPI (blue).
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682601 25 µg £70
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682602 100 µg £141
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Description

TOX is a member of the high-mobility group (HMG) box superfamily of DNA-binding proteins and is originally identified as the thymic transcript expressed in CD4+CD8+ double positive T-cells. TOX is also defined as part of a small subfamily of transcription factors consisting of TOX2, TOX3, and TOX4, which share conserved HMG-box sequences. TOX is highly upregulated in thymocytes undergoing positive selection and is required for the differentiation and development of various T-cell subsets, such as CD4+ T cells, regulatory T cells, and natural killer T cells (NKTs). TOX-deficient mice fail to develop lymph nodes and have significantly diminished numbers of CD4+ T-cells, NK cells, and ILC cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TOX recombinant protein (66-301 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The immunogen used to generate clone 6E6D03 is the N-terminus of Tox1, which is highly conserved among Tox family member proteins. Hence, this clone may recognize multiple Tox family members. Predicted MW for Tox family members: Tox 1 is 57.5 kD, Tox 2 is 49, 52, 53, 55 kD, Tox 3 is 63 kD and Tox 4 is 64, 66 kD.

Product Citations
  1. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID
AB_2566643 (BioLegend Cat. No. 682601)
AB_2566643 (BioLegend Cat. No. 682602)

Antigen Details

Structure
562 amino acids with a predicted molecular weight of 57.5 kD. Contains a high mobility group (HMG) box domain responsible for DNA binding.
Distribution

Nucleus.

Function
TOX is a transcription factor required for development of various T lymphocyte subsets.
Interaction
TOX interacts with ZDHHC17.
Biology Area
Cell Biology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Spits H. 2015. Nat. Immunol. 16:594.
2. Seehus CR, et al. 2015. Nat. Immunol. 16:599.
3. Huang Y, et al. 2015. Blood 125:1435.
4. Aliahmad P, et al. 2012. Curr. Opin. Immunol. 24:173.
6. Di Santo JP. 2010. Nat. Immunol. 11:885.
7. Aliahmad P, et al. 2004. J. Exp. Med. 199:1089.

Gene ID
9760 View all products for this Gene ID
UniProt
View information about TOX on UniProt.org
Go To Top Version: 2    Revision Date: 07/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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