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Purified anti-TPX2 Antibody

Pricing & Availability
Clone
18D5 (See other available formats)
Regulatory Status
RUO
Other Names
Targeting protein for Xklp1, restricted expression proliferation associated protein p100, differentially expressed in cancer and non-cancerous lung cells 2 (DIL2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_18D5_Purified_TPX2_Antibody_2_123118.png
Total cell lysates (15 µg total protein) from U87-MG, A431, HeLa, and NIH/3T3 cells were resolved by 4-20% Tris-Gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TPX2 Antibody, clone 18D5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower).
  • 1_18D5_Purified_TPX2_Antibody_2_123118.png
    Total cell lysates (15 µg total protein) from U87-MG, A431, HeLa, and NIH/3T3 cells were resolved by 4-20% Tris-Gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TPX2 Antibody, clone 18D5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:5000 dilution (lower).
  • 2_18D5_100405
    MOLT-4 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TPX2 (clone 18D5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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628002 100 µg £157
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Description

The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, α-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino Acid: 1-220 of human TPX2
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Fung TK, et al. 2007. Mol Biol Cell. 18:1861. PubMed
  2. Warner SL, et al. 2009. Clin Cancer Res. 15:6519. PubMed
  3. Bibby RA, et al. 2009. J. Biol Chem. PubMed
  4. Rohena CC, et al. 2013. Biochem Pharmacol. 85:1104. PubMed.
Product Citations
  1. Bibby R, et al. 2009. J Biol Chem. 284:33177. PubMed
  2. Warner S, et al. 2009. Clin Cancer Res. 5.152083333. PubMed
  3. Fung T, et al. 2007. Mol Biol Cell . 2.042361111. PubMed
  4. Eot-Houllier G, et al. 2010. J Biol Chem. 285:29556. PubMed
  5. Tagal V, et al. 2017. Nat Commun. 8:14098. PubMed
RRID
AB_2287863 (BioLegend Cat. No. 628002)

Antigen Details

Structure
Nuclear protein, contains two coiled-coil domains, approximately 100 kD
Distribution

Nuclear, during mitosis strictly associated with spindle pole and mitotic spindle. In G2/S cell cycle, diffusely distributed throughout nucleus. Highly expressed in lung carcinomas cell lines, but not in normal lung tissues.

Function
This protein is exclusively expressed in proliferating cells from the G1/S transition to the end of cytokinesis. Thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays. May also be involved in targeting Aurora-A kinase to spindle.
Interaction
Interacts with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1,α-tubulin, and nucleolin among others
Modification
Phosphorylation (S738)
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Gruss OJ, et al. 2002. Nature Cell Biol. 4:871.
2. Heidebrecht HJ, et al. 1997. Blood 90:226.
3. Kufer TA, et al. 2002. J. Cell Biol. 158:617.

Gene ID
22974 View all products for this Gene ID
UniProt
View information about TPX2 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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