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Purified anti-TRAF6 Antibody

Pricing & Availability
Clone
T2-1SC (See other available formats)
Regulatory Status
RUO
Other Names
TNF receptor-associated factor 6 (TRAF6), E3 ubiquitin protein ligase, Interleukin-1 signal transducer
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
T2-1SC_Purified_TRAF6_Antibody_WB_021813
Hela (Lane 1) and 3T3 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with a monoclonal anti-TRAF6 antibody. Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • T2-1SC_Purified_TRAF6_Antibody_WB_021813
    Hela (Lane 1) and 3T3 (Lane 2) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with a monoclonal anti-TRAF6 antibody. Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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654501 25 µg £109
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654502 100 µg £238
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Description

The protein encoded by this gene is a member of the TNF receptor-associated factor (TRAF) protein family. TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses. Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.

TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling as well as defects in neuronal development. Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities that result in signaling responses from the interleukin-1 receptor (IL-1R), toll-like receptor, CD40, RANK, and p75 neurotrophin receptor. TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK. This leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex. TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human TRAF6 protein (352-522 aa) expressed from E. coli.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
Affinity purified
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Tan Y, et al. 2019. Cell. 177:384. PubMed
  2. Ben J, et al. 2019. Nat Commun. 10:1801. PubMed
  3. Mitsumura T, et al. 2018. Blood Adv. 2.502083333. PubMed
RRID
AB_2561868 (BioLegend Cat. No. 654501)
AB_2561868 (BioLegend Cat. No. 654502)

Antigen Details

Structure
522 amino acids, predicted MW of 60 kD
Distribution
Cytoplasm, Nucleus
Function
A unique member of the TRAF family of adaptor proteins involved in both the TNF receptor superfamily and the interleukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily signal transduction pathways
Interaction
Homotrimer, homooligomer; Binds to TNFRSF5/CD40 and TNFRSF11A/RANK; Interacts with RBCK1 and TRAF3IP2
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
Antigen References

1. Rothe M, et al. 1994. Cell 78:681.
2. Arch RH, et al. 1998. Genes Dev. 12:2821.
3. Ye H, et al. 2002. Nature 418:443.
4. Yang WL, et al. 2009. Science 325:1134.
5. Qu F, et al. 2012. Mol. Cell. Biol. 32:3925.
6. Akiyama T, et al. 2012. Front Immunol. 3:278.
7. Wei J, et al. 2012. PLoS One 1:e49567.

Gene ID
7189 View all products for this Gene ID 22034 View all products for this Gene ID
UniProt
View information about TRAF6 on UniProt.org
Go To Top Version: 2    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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