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Purified anti-Transthyretin Antibody

Pricing & Availability
Clone
A16143A (See other available formats)
Regulatory Status
RUO
Other Names
TTR, ATTR, Prealbumin, TBPA, PALB
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16143A_Pure_Transthyretin_Antibody_1_102418
Western blot of purified anti-transthyretin antibody (clone A16143A). Lane 1: Molecular weight marker; Lane 2: 200 ng of human transthyretin recombinant protein; Lane 3: 4 µg of human plasma. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No.405306). Enhanced chemiluminescence was used as the detection system. Clone A16143A detects both TTR monomers (~16 kD) and oligomers in human plasma.
  • A16143A_Pure_Transthyretin_Antibody_1_102418
    Western blot of purified anti-transthyretin antibody (clone A16143A). Lane 1: Molecular weight marker; Lane 2: 200 ng of human transthyretin recombinant protein; Lane 3: 4 µg of human plasma. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No.405306). Enhanced chemiluminescence was used as the detection system. Clone A16143A detects both TTR monomers (~16 kD) and oligomers in human plasma.
  • A16143A_Pure_Transthyretin_Antibody_2_102418
    Western blot of purified anti-transthyretin antibody (clone A16143A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 20 µg of rat liver lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No.405306). Enhanced chemiluminescence was used as the detection system. Clone A16143A detects both TTR monomers (~16 kD) and oligomers in liver lysates.
  • A16143A_Pure_Transthyretin_Antibody_3_102418
    IHC staining of purified anti-transthyretin antibody (clone A16143A) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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856301 25 µg £77
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856302 100 µg £197
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Description

Transthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
TTR-KLH peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783454 (BioLegend Cat. No. 856301)
AB_2783454 (BioLegend Cat. No. 856302)

Antigen Details

Structure
Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 147 amino acids.
Distribution

Primarily extracellular in blood and cerebrospinal fluid.

Function
The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.
Interaction
Perlecan, receptor for advanced glycation end product (RAGE).
Cell Sources
Liver, choroid plexus epithelium, retina pigment epithelium.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Carrier Proteins
Antigen References
  1. Groenning M. et al. 2015. Sci. Rep. 5:11443.
  2. Saelices L. et al. 2015. J. Biol Chem. 290, 28932-43.
Gene ID
7276 View all products for this Gene ID
UniProt
View information about Transthyretin on UniProt.org
Go To Top Version: 1    Revision Date: 10/24/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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