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Purified anti-TRIP8b Antibody

Pricing & Availability
Clone
N212/17 (See other available formats)
Regulatory Status
RUO
Other Names
TPR-containing Rab8B-interacting protein, PEX5L, Tetratricopeptide Repeat-Containing Rab8b-Interacting Protein, HCN Channel Auxiliary Subunit, PEX5RP, PXR2B
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N212-17_PURE_TRIP8b_Antibody_1_101821.png
IHC staining of formalin-fixed paraffin-embedded human cerebellum stained with purified anti-TRIP8B (clone N212-17). Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong&tradel Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • N212-17_PURE_TRIP8b_Antibody_1_101821.png
    IHC staining of formalin-fixed paraffin-embedded human cerebellum stained with purified anti-TRIP8B (clone N212-17). Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (red) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong&tradel Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • N212-17_PURE_TRIP8b_Antibody_2_101821.png
    Whole cell extracts (15 µg total protein) from human, rat, and mouse brain membrane lysates were heated at 50°C for 10 minutes, resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified TRIP8b antibody (clone N212/17) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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601452 100 µg £113
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Description

TRIP8b is a protein that binds to and regulates hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, an important class of voltage-gated channels. Binding of TRIP8b to HCN channels causes them to open at more hyperpolarized potentials, as TRIP8b antagonizes the binding of cyclic nucleotides. Additionally, TRIP8b affects HCN channel subcellular localization. Via these functions, TRIP8b plays a role in learning and memory, major depressive disorder, and epilepsy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to the N-terminus of rat TRIP8b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Does not react with human in Western blot. Lysates were heated at 50°C for ten minutes prior to electrophoresis.

RRID
AB_2904443 (BioLegend Cat. No. 601452)

Antigen Details

Structure
TRIP8b is a 626 amino-acid protein with a predicted molecular weight of 70 kD.
Distribution

Expressed in the cell membrane and cytoplasm, with elevated expression in neurons and the parathyroid gland

Function
Regulates cell-surface localization and cyclic nucleotide dependence of HCN channels
Interaction
RAB8B, HCN1, HCN2, HCN3
Molecular Family
Postsynaptic proteins
Antigen References
  1. Han Y, et al. 2020. Channels (Austin). 14(1):110-122.
Gene ID
51555 View all products for this Gene ID
UniProt
View information about TRIP8b on UniProt.org
Go To Top Version: 1    Revision Date: 10/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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