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Purified anti-Tubulin α 8 Antibody

Pricing & Availability
Clone
P82D6D7 (See other available formats)
Regulatory Status
RUO
Other Names
α-tubulin 8, Tubulin α Chain-Like 2, TUBAL2, TUBA8
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
P82D6D7_PURE_Tubulin_alpha8_Antibody_1_WB_100716
Total cell lysate from HeLa (lane 1, 15 µg), MCF-7 (lane 2, 15 µg) and Jurkat (lane 3, 15 µg) were resolved by electrophoresis (4-12 % Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Tubulin α 8 antibody (clone P82D6D7). Proteins were visualized using a HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Purified anti-β-actin antibody (clone Poly6221) was used as a loading control.
  • P82D6D7_PURE_Tubulin_alpha8_Antibody_1_WB_100716
    Total cell lysate from HeLa (lane 1, 15 µg), MCF-7 (lane 2, 15 µg) and Jurkat (lane 3, 15 µg) were resolved by electrophoresis (4-12 % Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-Tubulin α 8 antibody (clone P82D6D7). Proteins were visualized using a HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Purified anti-β-actin antibody (clone Poly6221) was used as a loading control.
  • P82D6D7_PURE_Tubulin_alpha8_Antibody_2_ICC_041519
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL anti-Tubulin α 8 (clone P82D6D7) in blocking buffer overnight at 4°C and followed by DyLight™ 594 (red) conjugated goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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691702 100 µg £174
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Description

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta tubulin. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. Tubulin α 8 belongs to the Tubulin α family which contains 8 other members such as tubulin α 1A, tubulin α 4A, tubulin α 3E. It has been shown that mutations in the tubulin α 8 gene is associated with polymicrogyria and optic nerve hypoplasia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human Tubulin α 8 (a.a. 294-449) expressed in E. coli. This region shares high percentage of homology with other Tubulin α members.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone P82D6D7 may also recognize the other Tubulin a members since this clone was developed with partial sequence of tubulin a 8 (a.a. 294-449) that shares high percentage of homology with other members in the Tubulin a family such as tubulin a 4A, tubulin a 3C/D, and tubulin a 1A.

RRID
AB_2632654 (BioLegend Cat. No. 691702)

Antigen Details

Structure
Tubulin α 8 contains 449 amino acids with a predicted molecular weight of 50 kD.
Distribution

Cytoskeleton/Ubiquitous.

Function
Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site.
Interaction
RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738.
2. Miller FD, et al. 1987. J. Cell. Biol. 105:3065.
3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.
4. Abdollahi MR, et al. 2009. Am. J. Hum. Genet. 85:737.

Gene ID
51807 View all products for this Gene ID
UniProt
View information about Tubulin alpha 8 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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