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Purified anti-Tubulin-β 2B (TUBB2B) Antibody (Previously Covance catalog# MMS-422P)

Pricing & Availability
Clone
7B9 (See other available formats)
Regulatory Status
RUO
Other Names
Tubulin beta-2B chain, class IIb beta-tubulin, class II beta-tubulin isotype, tubulin, beta polypeptide paralog
Previously
Covance Catalog# MMS-422P
Isotype
Mouse IgG3
Ave. Rating
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Product Citations
publications
1_7B9_Pure_Tubulin-B-2B_Antibody_1_071018
Western blot of purified anti-Tubulin-β 2B (TUBB2B) antibody (clone 7B9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_7B9_Pure_Tubulin-B-2B_Antibody_1_071018
    Western blot of purified anti-Tubulin-β 2B (TUBB2B) antibody (clone 7B9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_7B9_Pure_Tubulin-B-2B_Antibody_2_071018
    ICC staining of purified anti-Tubulin-β 2B (TUBB2B) antibody (clone 7B9) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 5 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with 5 µg/ml of Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_7B9_PURE_TUBB2B_Antibody_Xenopus-laevis_040919
    Xenopus embryos were immunostained with anti-Tubulin-β 2B (TUBB2B) antibody (1:50 dilution, BioLegend Cat. No. 909101), followed by Alexa Fluor® 488 goat anti-mouse IgG (Cat. No. 405319). Immunostained embryos were cleared in BBBA (2:1 benzyl benzoate/benzyl alcohol) and fixed for confocal imaging. Data provided by Dr. Daniel Levy and Richik Mukherjee at the University of Wyoming.
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909101 200 µL £241
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Description

Tubulin is a major component of microtubules. It exists as a heterodimer consiting of an alpha and a beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons, although it is not neuronal specific and is found in many other tissues including lung tissue and Schwann cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Frog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against the synthetic peptide CEEEEGEDEA found at the c-terminus of beta II tubulin.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC).
 

Xenopus reactivity was verified by Dr. Daniel Levy’s lab at the University of Wyoming 

Application References

(PubMed link indicates BioLegend citation)
  1. Moody SA, et al. 1996. J. Comp. Neuro. 364: 219.
  2. Dozier JH, et al. 2003. Breast Cancer Res. 5:R157.
Product Citations
  1. Jevtic P, et al. 2019. PLoS One. 22:e0215740. PubMed
RRID
AB_2565093 (BioLegend Cat. No. 909101)

Antigen Details

Structure
Expected MW: 50 kD
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Gene ID
347733 View all products for this Gene ID
UniProt
View information about Tubulin-beta 2B on UniProt.org
Go To Top Version: 7    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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