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Purified anti-Tubulin-γ Antibody

Pricing & Availability
Clone
14C11 (See other available formats)
Regulatory Status
RUO
Other Names
γ-tubulin
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_14C11_PURE_Tubulin_gamma_Antibody_WB_121217
Total lysates (15 µg protein) from HaCaT, HeLa (Human) and Raw264.7 (Mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-Tubulin-γ antibody, clone 14C11. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat.No. 643807, 1:8000 dilution).
  • 1_14C11_PURE_Tubulin_gamma_Antibody_WB_121217
    Total lysates (15 µg protein) from HaCaT, HeLa (Human) and Raw264.7 (Mouse) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/ml) purified anti-Tubulin-γ antibody, clone 14C11. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat.No. 643807, 1:8000 dilution).
  • 2_14C11_PURE_Tubulin_gamma_antibody_2_ICC_040419
    Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against g-Tubulin (clone 14C11) followed by Rhodamine Red-X conjugated donkey anti-mouse IgG and DAPI.
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629201 25 µg £70
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Description

γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide-KLH
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:500-1:1000). For immunocytochemistry, a concentration of 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse, and rat ?-tubulin.

Product Citations
  1. Amo L, et al. 2022. Front Immunol. 13:835527. PubMed
  2. Natalie Lerner, Sofia Avissar, Elie Beit-Yannai 2017. PLoS One. 12(2):e0171153. PubMed
RRID
AB_493419 (BioLegend Cat. No. 629201)

Antigen Details

Structure
Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution

Cytoskeletal protein, high expressed in centrosomes

Function
Cytoskeletal organization, mitosis
Interaction
Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Zheng Y, et al. 1991. Cell 65:817.

Regulation
Expressed at highest levels during prophase to metaphase
Gene ID
2776 View all products for this Gene ID
UniProt
View information about Tubulin-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/12/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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