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Purified anti-Ubiquitin Antibody

Pricing & Availability
Clone
P4D1 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P4D1_DBHRP_Ubiquitin_Antibody_WB_1_092920
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated with 10 µM MG-132 for 10 hours (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.125 µg/mL (1:4000 dilution) of purified anti-Ubiquitin antibody (clone P4D1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular weight marker.
  • P4D1_DBHRP_Ubiquitin_Antibody_WB_1_092920
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated with 10 µM MG-132 for 10 hours (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.125 µg/mL (1:4000 dilution) of purified anti-Ubiquitin antibody (clone P4D1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular weight marker.
  • P4D1_DBHRP_Ubiquitin_Antibody_WB_2_092920
    Mouse recombinant ubiquitin was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Ubiquitin antibody, clone P4D1. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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646301 25 µg £85
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646302 100 µg £182
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Description

Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more than one ubiquitin monomers. Ubiqutination controls the protein degradation, stability and intracellular localization of many proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Denatured glutaraldehyde cross-linked ubiquitin IgG complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.12 to 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.

Application References

(PubMed link indicates BioLegend citation)
  1. Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770. PubMed.
Product Citations
  1. Zhang A, et al. 2022. EMBO J. 41:e109187. PubMed
  2. Mennerich D, et al. 2022. Redox Biol. 50:102243. PubMed
  3. Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed
  4. Singh F, et al. 2021. Elife. 10:. PubMed
  5. Schreiner J, et al. 2013. Neurobiol Aging. 34:770. PubMed
  6. De Cesare V, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  7. Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed
  8. Kanamori T, et al. 2021. Sci Rep. 11:14726. PubMed
  9. Deguise M, et al. 2016. Sci Rep. 6:28846. PubMed
  10. Harris IS et al. 2019. Cell metabolism. 29(5):1166-1181 . PubMed
  11. Liu X, et al. 2022. J Am Chem Soc. :. PubMed
  12. Feng T, et al. 2020. EMBO Rep. 21:e50219. PubMed
  13. Bermudez-Brito M, Faas M, Vos P 2016. Nature. 10.1038/nature18943. PubMed
  14. Abreu AP, et al. 2020. J Clin Invest. 130:4486. PubMed
  15. Martins-Marques T, et al. 2020. Life Sci Alliance. 3:00. PubMed
  16. Malik AU, et al. 2021. Biochem J. 478:553. PubMed
RRID
AB_1659269 (BioLegend Cat. No. 646301)
AB_1659269 (BioLegend Cat. No. 646302)

Antigen Details

Structure
Consists of 76 amino acids and has a molecular mass of about 8.5 kd. Highly conserved among all eukaryotic species.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Autophagosome Markers
Antigen References

1. Kahana, et al. 1999. Mol. Cell Biol 19:6608
2. Ciechanover A, et al. 1998. EMBO J 17:7151
3. Xu P, et al. 2008. Analytical Chemistry 80:3438

Gene ID
6233 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org
Go To Top Version: 3    Revision Date: 09/29/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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